| IED ID | IndEnz0002004274 |
| Enzyme Type ID | protease004274 |
| Protein Name |
Ovoinhibitor OI OvoI Serine protease inhibitor Kazal-type 5 allergen Gal d OIH |
| Gene Name | SPINK5 OIH |
| Organism | Gallus gallus (Chicken) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Archelosauria Archosauria Dinosauria Saurischia Theropoda Coelurosauria Aves Neognathae Galloanserae Galliformes Phasianidae (turkeys) Phasianinae Gallus Gallus gallus (Chicken) |
| Enzyme Sequence | MTDWVLHHKVGPLDMTTRYIFPLLPLPFLPHSESKRAVCAPRCSAMRTARQFVQVALALCCFADIAFGIEVNCSLYASGIGKDGTSWVACPRNLKPVCGTDGSTYSNECGICLYNREHGANVEKEYDGECRPKHVTIDCSPYLQVVRDGNTMVACPRILKPVCGSDSFTYDNECGICAYNAEHHTNISKLHDGECKLEIGSVDCSKYPSTVSKDGRTLVACPRILSPVCGTDGFTYDNECGICAHNAEQRTHVSKKHDGKCRQEIPEIDCDQYPTRKTTGGKLLVRCPRILLPVCGTDGFTYDNECGICAHNAQHGTEVKKSHDGRCKERSTPLDCTQYLSNTQNGEAITACPFILQEVCGTDGVTYSNDCSLCAHNIELGTSVAKKHDGRCREEVPELDCSKYKTSTLKDGRQVVACTMIYDPVCATNGVTYASECTLCAHNLEQRTNLGKRKNGRCEEDITKEHCREFQKVSPICTMEYVPHCGSDGVTYSNRCFFCNAYVQSNRTLNLVSMAAC |
| Enzyme Length | 517 |
| Uniprot Accession Number | P10184 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Serine protease inhibitor involved in antimicrobial egg defense preventing contamination of table eggs (non-fertilized eggs) and protecting the chick embryo (fertilized eggs) (Probable). Inhibits trypsin, chymotrypsin, elastase, subtilisin and a proteinase of fungus Aspergillus oryzae (PubMed:13944692, PubMed:6904299, PubMed:6838526, PubMed:14519973, PubMed:14609095, PubMed:22010862). Inhibits calcium-activated potassium channels KCNMA1 (bovine) and slo (Drosophila) (PubMed:8973172). Has antibacterial activity against B.thuringiensis LMSA 3.06.004, but not against S.aureus CIP 103 811, P.aeruginosa PAO1, B.cereus ATCC6464 or B.subtilis ATCC 6633 (PubMed:22010862). {ECO:0000269|PubMed:13944692, ECO:0000269|PubMed:14519973, ECO:0000269|PubMed:14609095, ECO:0000269|PubMed:22010862, ECO:0000269|PubMed:6838526, ECO:0000269|PubMed:6904299, ECO:0000269|PubMed:8973172, ECO:0000305|PubMed:22010862}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (1); Chain (1); Disulfide bond (21); Domain (7); Erroneous initiation (1); Glycosylation (3); Sequence conflict (4); Site (7) |
| Keywords | Allergen;Alternative splicing;Antimicrobial;Direct protein sequencing;Disulfide bond;Glycoprotein;Protease inhibitor;Reference proteome;Repeat;Secreted;Serine protease inhibitor |
| Interact With | P00766 |
| Induction | INDUCTION: Expression is regulated by dietary stress. Significantly increased expression between days 0 to 5 in egg whites of eggs laid by corticosterone-fed hens (at protein level). Decreased expression at day 14 in the magnum of the oviduct in the corticosterone-fed laying hens (PubMed:25436390). Significantly increased expression by estrogen. Rapidly up-regulated within 0.5 hour after extrogen exposure with a peak at 1-4 hours and diminishing thereafter (PubMed:11572089). Up-regulated during sexual maturation of pullets (PubMed:22010862). {ECO:0000269|PubMed:11572089, ECO:0000269|PubMed:22010862, ECO:0000269|PubMed:25436390}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22010862}. |
| Modified Residue | |
| Post Translational Modification | PTM: Glycosylated. {ECO:0000269|PubMed:3025257}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 30608187; |
| Motif | |
| Gene Encoded By | |
| Mass | 57,005 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |