| IED ID | IndEnz0002004379 |
| Enzyme Type ID | protease004379 |
| Protein Name |
Putative GPI-anchor transamidase GPI transamidase EC 3.-.-.- Hypersensitive to pore-forming toxin protein 4 Phosphatidylinositol-glycan biosynthesis class K protein PIG-K |
| Gene Name | pigk-1 hpo-4 T05E11.6 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MRHVLLIFCAIIATEALLNTGLQLKIDELFDTPGHTNNWAVLVCTSKFWFNYRHVSNVLALYHSIKRLGIPDSNIIMMLAEDVPCNSRNPRPGTVYAARAGTNLYGSDVEVDYRGEEVTVESFIRVLTGRHHPATPRSKRLLTDHQSNVLIYLTGHGGDSFMKFQDSEELTNVDLAYAIQTMFEDNRYHEMLVIADSCRSASMYEWIDSPNVLSLSSSLTHEESYSYDVDTDIGVYVIDRYTHYTVNFLTKEVKALNSSANMQDYIDSCPARKCLSNTGVRKDHYPKDVKRVRVTDFFGSSRIFQHLSEEIVLDDEFWA |
| Enzyme Length | 319 |
| Uniprot Accession Number | P49048 |
| Absorption | |
| Active Site | ACT_SITE 156; /evidence=ECO:0000250; ACT_SITE 198; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.-.-.- |
| Enzyme Function | FUNCTION: Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis. |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Glycosylation (1); Signal peptide (1) |
| Keywords | GPI-anchor biosynthesis;Glycoprotein;Hydrolase;Protease;Reference proteome;Signal;Thiol protease |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 21177967; 21408619; 22286215; 22298425; 22560298; 23800452; 25487147; |
| Motif | |
| Gene Encoded By | |
| Mass | 36,417 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |