| IED ID | IndEnz0002004380 |
| Enzyme Type ID | protease004380 |
| Protein Name |
GPI-anchor transamidase GPI transamidase EC 3.-.-.- |
| Gene Name | gpi8 SPCC11E10.02c |
| Organism | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota Taphrinomycotina Schizosaccharomycetes Schizosaccharomycetales Schizosaccharomycetaceae Schizosaccharomyces Schizosaccharomyces pombe (Fission yeast) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Enzyme Sequence | MIVQFVALLLLNLLQIIAAESSHTNNWAVLISTSRFWFNYRHTANVLGIYRSVKRLGIPDSQIILMIADDYACNSRNLFPGTVFDNADRALDLYGEEIEIDYKGYEVTVEAFIRLLTERVPENTPASKRLLTNERSNILIYMTGHGGDGFIKFQDAEELSSEDLADAIEQIHQHKRYNEILFMVDTCQANSLYTKIYSPNVLAIGSSEVGTSSYSHHADIDIGVAVIDRFTFSNLEFLENRVDSKSKLTMQDLINSYNPYEIHSTPGVQPINLRRSPDDILITDFFGNVRDIELHSEKINWMLPGENTTKPSIKRNSFVFQAQNDMQDDGKGFGISNLKSFLPPTRELKYKKHPISRIISAVVCISFSIGFPYYASKYLK |
| Enzyme Length | 380 |
| Uniprot Accession Number | Q9USP5 |
| Absorption | |
| Active Site | ACT_SITE 145; /evidence=ECO:0000250; ACT_SITE 187; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.-.-.- |
| Enzyme Function | FUNCTION: Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis. |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (1); Glycosylation (1); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | Disulfide bond;Endoplasmic reticulum;GPI-anchor biosynthesis;Glycoprotein;Hydrolase;Membrane;Protease;Reference proteome;Signal;Thiol protease;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | PTM: The disulfide bond between PIGK/GPI8 and PIGT is important for normal enzyme activity. {ECO:0000250}. |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 20473289; 22633491; 23697806; |
| Motif | |
| Gene Encoded By | |
| Mass | 43,206 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |