Detail Information for IndEnz0002004426
IED ID IndEnz0002004426
Enzyme Type ID protease004426
Protein Name Neutral protease NprE
EC 3.4.24.28
Bacillolysin-like protease
Milk-clotting protease from Bacillus mesentericus strain 76
MCP 76
Gene Name nprE
Organism Bacillus pumilus (Bacillus mesentericus)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus pumilus (Bacillus mesentericus)
Enzyme Sequence AAATGSGTTLKGATVPLNISYEGGKYVLRDLSKPTGTQIITYDLQNRQSRLPGTLVSSTTKTFTSSSQRAAVDAHYNLGKVYDYFYSNFKRNSYDNKGSKIVSSVHYGTQYNNAAWTGDQMIYGDGDGSFFSPLSGSLDVTAHEMTHGVTQETANLIYENQPGALNESFSDVFGYFNDTEDWDIGEDITVSQPALRSLSNPTKYNQPDNYANYRNLPNTDEGDYGGVHTNSGIPNKAAYNTITKLGVSKSQQIYYRALTTYLTPSSTFKDAKAALIQSARDLYGSTDAAKVEAAWNAVGL
Enzyme Length 300
Uniprot Accession Number P68734
Absorption
Active Site ACT_SITE 144; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; ACT_SITE 228; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Similar, but not identical, to that of thermolysin.; EC=3.4.24.28;
DNA Binding
EC Number 3.4.24.28
Enzyme Function FUNCTION: Extracellular zinc metalloprotease.
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Thermolabile.;
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (11)
Keywords Calcium;Direct protein sequencing;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Zinc
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 32,674
Kinetics
Metal Binding METAL 139; /note=Calcium 1; /evidence=ECO:0000255; METAL 143; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 147; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 167; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 178; /note=Calcium 1; /evidence=ECO:0000255; METAL 178; /note=Calcium 2; /evidence=ECO:0000255; METAL 181; /note=Calcium 1; /evidence=ECO:0000255; METAL 181; /note=Calcium 2; /evidence=ECO:0000255; METAL 183; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000255; METAL 186; /note=Calcium 1; /evidence=ECO:0000255; METAL 186; /note=Calcium 2; /evidence=ECO:0000255
Rhea ID
Cross Reference Brenda