IED ID | IndEnz0002004426 |
Enzyme Type ID | protease004426 |
Protein Name |
Neutral protease NprE EC 3.4.24.28 Bacillolysin-like protease Milk-clotting protease from Bacillus mesentericus strain 76 MCP 76 |
Gene Name | nprE |
Organism | Bacillus pumilus (Bacillus mesentericus) |
Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus pumilus (Bacillus mesentericus) |
Enzyme Sequence | AAATGSGTTLKGATVPLNISYEGGKYVLRDLSKPTGTQIITYDLQNRQSRLPGTLVSSTTKTFTSSSQRAAVDAHYNLGKVYDYFYSNFKRNSYDNKGSKIVSSVHYGTQYNNAAWTGDQMIYGDGDGSFFSPLSGSLDVTAHEMTHGVTQETANLIYENQPGALNESFSDVFGYFNDTEDWDIGEDITVSQPALRSLSNPTKYNQPDNYANYRNLPNTDEGDYGGVHTNSGIPNKAAYNTITKLGVSKSQQIYYRALTTYLTPSSTFKDAKAALIQSARDLYGSTDAAKVEAAWNAVGL |
Enzyme Length | 300 |
Uniprot Accession Number | P68734 |
Absorption | |
Active Site | ACT_SITE 144; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; ACT_SITE 228; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Similar, but not identical, to that of thermolysin.; EC=3.4.24.28; |
DNA Binding | |
EC Number | 3.4.24.28 |
Enzyme Function | FUNCTION: Extracellular zinc metalloprotease. |
Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Thermolabile.; |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (2); Chain (1); Metal binding (11) |
Keywords | Calcium;Direct protein sequencing;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Zinc |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 32,674 |
Kinetics | |
Metal Binding | METAL 139; /note=Calcium 1; /evidence=ECO:0000255; METAL 143; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 147; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 167; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 178; /note=Calcium 1; /evidence=ECO:0000255; METAL 178; /note=Calcium 2; /evidence=ECO:0000255; METAL 181; /note=Calcium 1; /evidence=ECO:0000255; METAL 181; /note=Calcium 2; /evidence=ECO:0000255; METAL 183; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000255; METAL 186; /note=Calcium 1; /evidence=ECO:0000255; METAL 186; /note=Calcium 2; /evidence=ECO:0000255 |
Rhea ID | |
Cross Reference Brenda |