| IED ID | IndEnz0002004430 |
| Enzyme Type ID | protease004430 |
| Protein Name |
Subtilisin-like serine protease Pen ch 13 EC 3.4.21.- Alkaline serine protease allergen Pen ch 13 |
| Gene Name | |
| Organism | Penicillium rubens |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Penicillium Penicillium chrysogenum species complex Penicillium rubens |
| Enzyme Sequence | MGFLKLLSTSLATLAVVNAGKLLTANDGDEVVPSSYIVVMNDGVSTAQFETHRNWAANVHARTRSLKGGESGPGKHFDINGMKGYSASFDDRTVKDIASDPTVKYVEPDMVVNATANVVQRNAPSWGLSRISSKKSGATDYVYDSTAGEGIVIYGVDTGIDIGHADFGGRAEWGTNTADNDDTDGNGHGTHTASTAAGSKFGVAKKASVVAVKVLGADGSGTNSQVIAGMDWAVKDSKSRGATGKSVMNMSLGGAYSRAMNDAAANVVRSGVFLSVAAGNEAQDASNSSPASAPNVCTIAASTNSDGSASFTNFGSVVDLYAPGKDITAAYPGGGSKTLSGTSMAAPHVAGAAAYLMALEGVTSDKACARIVELAISSISSAPSGTTSKLLYNGINAQ |
| Enzyme Length | 398 |
| Uniprot Accession Number | P9WEW3 |
| Absorption | |
| Active Site | ACT_SITE 157; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 188; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 343; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by phenylmethanesulfonyl fluoride (PMSF) and diethyl pyrocarbonate (DEPC), but not by benzamidine. {ECO:0000269|PubMed:11893850}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Serine protease (PubMed:11893850, PubMed:16436150). Hydrolyzes casein, gelatin and human collagen type IV, but not elastin in vitro (PubMed:16436150). Hydrolyzes OCLN of the human lung epithelial cells at 202-Gln-|-Ser-203 and Gln-211-|-Ile-212 (PubMed:16436150). {ECO:0000269|PubMed:11893850, ECO:0000269|PubMed:16436150}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Active at neutral to pH 8 with casein as substrate. {ECO:0000269|PubMed:11893850}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Compositional bias (1); Domain (2); Glycosylation (2); Mutagenesis (3); Propeptide (1); Region (5); Signal peptide (1); Site (1) |
| Keywords | Allergen;Direct protein sequencing;Glycoprotein;Hydrolase;IgE-binding protein;Protease;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11893850, ECO:0000269|PubMed:12602675, ECO:0000269|PubMed:16436150}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 40,362 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |