| IED ID | IndEnz0002004468 |
| Enzyme Type ID | protease004468 |
| Protein Name |
Carboxypeptidase G2 CPDG2 EC 3.4.17.11 Folate hydrolase G2 Glutamate carboxypeptidase Pteroylmonoglutamic acid hydrolase G2 Glucarpidase |
| Gene Name | cpg2 |
| Organism | Pseudomonas sp. (strain RS-16) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Proteobacteria incertae sedis unclassified pseudomonads Pseudomonas sp. (strain RS-16) |
| Enzyme Sequence | MRPSIHRTAIAAVLATAFVAGTALAQKRDNVLFQAATDEQPAVIKTLEKLVNIETGTGDAEGIAAAGNFLEAELKNLGFTVTRSKSAGLVVGDNIVGKIKGRGGKNLLLMSHMDTVYLKGILAKAPFRVEGDKAYGPGIADDKGGNAVILHTLKLLKEYGVRDYGTITVLFNTDEEKGSFGSRDLIQEEAKLADYVLSFEPTSAGDEKLSLGTSGIAYVQVNITGKASHAGAAPELGVNALVEASDLVLRTMNIDDKAKNLRFNWTIAKAGNVSNIIPASATLNADVRYARNEDFDAAMKTLEERAQQKKLPEADVKVIVTRGRPAFNAGEGGKKLVDKAVAYYKEAGGTLGVEERTGGGTDAAYAALSGKPVIESLGLPGFGYHSDKAEYVDISAIPRRLYMAARLIMDLGAGK |
| Enzyme Length | 415 |
| Uniprot Accession Number | P06621 |
| Absorption | |
| Active Site | ACT_SITE 114; /evidence=ECO:0000250; ACT_SITE 175; /note=Proton acceptor; /evidence=ECO:0000269|PubMed:9083113 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of C-terminal glutamate residues from a wide range of N-acylating moieties, including peptidyl, aminoacyl, benzoyl, benzyloxycarbonyl, folyl and pteroyl groups.; EC=3.4.17.11; |
| DNA Binding | |
| EC Number | 3.4.17.11 |
| Enzyme Function | FUNCTION: Catalyzes the hydrolysis of reduced and non-reduced folates to pteroates and L-glutamate. This enzyme has a broad specificity. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (16); Chain (1); Helix (15); Metal binding (6); Signal peptide (1); Turn (3) |
| Keywords | 3D-structure;Carboxypeptidase;Direct protein sequencing;Hydrolase;Metal-binding;Metalloprotease;Pharmaceutical;Protease;Signal;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..22 |
| Structure 3D | X-ray crystallography (3) |
| Cross Reference PDB | 1CG2; 6XJ5; 7M6U; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 43,932 |
| Kinetics | |
| Metal Binding | METAL 112; /note=Zinc 2; /evidence=ECO:0000269|PubMed:9083113; METAL 141; /note=Zinc 1; /evidence=ECO:0000269|PubMed:9083113; METAL 141; /note=Zinc 2; /evidence=ECO:0000269|PubMed:9083113; METAL 176; /note=Zinc 1; /evidence=ECO:0000269|PubMed:9083113; METAL 200; /note=Zinc 2; /evidence=ECO:0000269|PubMed:9083113; METAL 385; /note=Zinc 1; /evidence=ECO:0000269|PubMed:9083113 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.17.11; |