IED Industry Enzyme Database

Detail Information for IndEnz0002004538
IED ID IndEnz0002004538
Enzyme Type ID protease004538
Protein Name Collagen alpha-1
I
chain
Alpha-1 type I collagen
Gene Name Col1a1
Organism Rattus norvegicus (Rat)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat)
Enzyme Sequence MFSFVDLRLLLLLGATALLTHGQEDIPEVSCIHNGLRVPNGETWKPDVCLICICHNGTAVCDGVLCKEDLDCPNPQKREGECCPFCPEEYVSPDAEVIGVEGPKGDPGPQGPRGPVGPPGQDGIPGQPGLPGPPGPPGPPGPPGLGGNFASQMSYGYDEKSAGVSVPGPMGPSGPRGLPGPPGAPGPQGFQGPPGEPGEPGASGPMGPRGPPGPPGKNGDDGEAGKPGRPGERGPPGPQGARGLPGTAGLPGMKGHRGFSGLDGAKGDTGPAGPKGEPGSPGENGAPGQMGPRGLPGERGRPGPPGSAGARGNDGAVGAAGPPGPTGPTGPPGFPGAAGAKGEAGPQGARGSEGPQGVRGEPGPPGPAGAAGPAGNPGADGQPGAKGANGAPGIAGAPGFPGARGPSGPQGPSGAPGPKGNSGEPGAPGNKGDTGAKGEPGPAGVQGPPGPAGEEGKRGARGEPGPSGLPGPPGERGGPGSRGFPGADGVAGPKGPAGERGSPGPAGPKGSPGEAGRPGEAGLPGAKGLTGSPGSPGPDGKTGPPGPAGQDGRPGPAGPPGARGQAGVMGFPGPKGTAGEPGKAGERGVPGPPGAVGPAGKDGEAGAQGAPGPAGPAGERGEQGPAGSPGFQGLPGPAGPPGEAGKPGEQGVPGDLGAPGPSGARGERGFPGERGVQGPPGPAGPRGNNGAPGNDGAKGDTGAPGAPGSQGAPGLQGMPGERGAAGLPGPKGDRGDAGPKGADGSPGKDGVRGLTGPIGPPGPAGAPGDKGETGPSGPAGPTGARGAPGDRGEPGPPGPAGFAGPPGADGQPGAKGEPGDTGVKGDAGPPGPAGPAGPPGPIGNVGAPGPKGSRGAAGPPGATGFPGAAGRVGPPGPSGNAGPPGPPGPVGKEGGKGPRGETGPAGRPGEVGPPGPPGPAGEKGSPGADGPAGSPGTPGPQGIAGQRGVVGLPGQRGERGFPGLPGPSGEPGKQGPSGASGERGPPGPMGPPGLAGPPGESGREGSPGAEGSPGRDGAPGAKGDRGETGPAGPPGAPGAPGAPGPVGPAGKNGDRGETGPAGPAGPIGPAGARGPAGPQGPRGDKGETGEQGDRGIKGHRGFSGLQGPPGSPGSPGEQGPSGASGPAGPRGPPGSAGSPGKDGLNGLPGPIGPPGPRGRTGDSGPAGPPGPPGPPGPPGPPSGGYDFSFLPQPPQEKSQDGGRYYRADDANVVRDRDLEVDTTLKSLSQQIENIRSPEGSRKNPARTCRDLKMCHSDWKSGEYWIDPNQGCNLDAIKVYCNMETGQTCVFPTQPSVPQKNWYISPNPKEKKHVWFGESMTDGFQFEYGSEGSDPADVAIQLTFLRLMSTEASQNITYHCKNSVAYMDQQTGNLKKSLLLQGSNEIELRGEGNSRFTYSTLVDGCTSHTGTWGKTVIEYKTTKTSRLPIIDVAPLDIGAPDQEFGMDIGPACFV
Enzyme Length 1453
Uniprot Accession Number P02454
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Type I collagen is a member of group I collagen (fibrillar forming collagen).
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Compositional bias (6); Disulfide bond (5); Domain (2); Glycosylation (4); Metal binding (5); Modified residue (125); Motif (2); Propeptide (2); Region (5); Sequence conflict (18); Signal peptide (1)
Keywords 3D-structure;Calcium;Collagen;Direct protein sequencing;Disulfide bond;Extracellular matrix;Glycoprotein;Hydroxylation;Metal-binding;Phosphoprotein;Pyrrolidone carboxylic acid;Reference proteome;Repeat;Secreted;Signal
Interact With Q9UBG0
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix {ECO:0000255|PROSITE-ProRule:PRU00793}.
Modified Residue MOD_RES 152; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000269|PubMed:5777344; MOD_RES 160; /note=Allysine; /evidence=ECO:0000269|PubMed:5777344; MOD_RES 161; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:22673903; MOD_RES 179; /note=4-hydroxyproline; /evidence=ECO:0000305|PubMed:4290711; MOD_RES 182; /note=4-hydroxyproline; /evidence=ECO:0000305|PubMed:4290711; MOD_RES 185; /note=4-hydroxyproline; /evidence=ECO:0000305|PubMed:4290711; MOD_RES 194; /note=4-hydroxyproline; /evidence=ECO:0000305|PubMed:4290711; MOD_RES 197; /note=4-hydroxyproline; /evidence=ECO:0000305|PubMed:4290711; MOD_RES 200; /note=4-hydroxyproline; /evidence=ECO:0000305|PubMed:4290711; MOD_RES 215; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 230; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 236; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 245; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 251; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 254; /note=5-hydroxylysine; alternate; /evidence=ECO:0000269|PubMed:5411206; MOD_RES 260; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:22673903; MOD_RES 278; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 281; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 287; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 296; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 302; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 323; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 332; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 335; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 362; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 365; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 377; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 383; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 392; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 398; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 401; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 416; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 419; /note=5-hydroxylysine; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 425; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 428; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 440; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 449; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 464; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 470; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 479; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 485; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 494; /note=5-hydroxylysine; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 503; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 512; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 518; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 524; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 533; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 536; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 545; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 554; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 560; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 572; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 581; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 590; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 593; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 611; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 629; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 635; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 641; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 647; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 653; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 659; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 671; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 680; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 692; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 704; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 707; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 713; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 719; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 728; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 740; /note=5-hydroxylysine; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 746; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 761; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 767; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 776; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:22673903; MOD_RES 788; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 794; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 797; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 806; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 812; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 830; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 839; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 848; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 851; /note=5-hydroxylysine; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 860; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 866; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 874; /note=3-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P11087; MOD_RES 875; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P11087; MOD_RES 884; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P11087; MOD_RES 887; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P11087; MOD_RES 908; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 917; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 926; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 935; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 953; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 962; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 965; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 971; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 986; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 992; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 998; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 1007; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 1013; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 1022; /note=5-hydroxylysine; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 1034; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 1037; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 1040; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 1085; /note=5-hydroxylysine; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 1097; /note=5-hydroxylysine; alternate; /evidence=ECO:0000250|UniProtKB:P11087; MOD_RES 1109; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 1112; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 1115; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 1133; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P02457; MOD_RES 1148; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P11087; MOD_RES 1153; /note=3-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P11087; MOD_RES 1154; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P11087; MOD_RES 1168; /note=3-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P11087; MOD_RES 1169; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P11087; MOD_RES 1171; /note=3-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P11087; MOD_RES 1172; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P11087; MOD_RES 1174; /note=3-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P11087; MOD_RES 1175; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P11087; MOD_RES 1178; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P11087; MOD_RES 1181; /note=4-hydroxyproline; /evidence=ECO:0000250|UniProtKB:P11087; MOD_RES 1197; /note=Allysine; /evidence=ECO:0000250|UniProtKB:P02453
Post Translational Modification PTM: Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. {ECO:0000269|PubMed:5411206}.; PTM: Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline. {ECO:0000250|UniProtKB:P11087}.; PTM: Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains. {ECO:0000250|UniProtKB:P02457}.; PTM: O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide. {ECO:0000269|PubMed:5411206}.
Signal Peptide SIGNAL 1..22; /evidence=ECO:0000255
Structure 3D Fiber diffraction (2)
Cross Reference PDB 3HQV; 3HR2;
Mapped Pubmed ID 10051504; 10679825; 10970885; 11748224; 11918316; 11924646; 12199527; 12297293; 12351664; 12408871; 12412812; 12647294; 12755333; 14595530; 14749390; 14993121; 15042712; 15552839; 15609627; 15647278; 15677443; 15703183; 16009107; 16306131; 16396496; 16428894; 16938497; 16971509; 16972247; 17156988; 17206378; 17241878; 17485223; 17485851; 17616939; 17924680; 17977875; 18041732; 18348167; 18471258; 18726071; 18727865; 18782537; 18988763; 19019833; 1921334; 19362560; 19393425; 19418583; 19426591; 19562802; 19667256; 19679517; 20006603; 20056896; 20149607; 2016852; 20388018; 20397200; 20479531; 20647009; 20660018; 20818932; 20875682; 21055467; 21093551; 21166192; 21181362; 21274875; 21295105; 21645221; 21729992; 21769867; 21842416; 21926544; 22206666; 22615126; 22706148; 22824087; 22847422; 22903132; 23069578; 23093703; 23313213; 23508044; 23560091; 23633075; 23722449; 23958495; 23959432; 24036265; 24116223; 2456904; 24735795; 25128628; 25636075; 25655816; 25784725; 25867313; 26049074; 26097527; 26481812; 26567337; 26578432; 2659889; 26674152; 26702050; 26807691; 27031437; 27108411; 27155082; 27351590; 27756197; 28031326; 28713909; 28731181; 28849131; 29286102; 29733741; 31186140; 32328952; 33497804; 7511187;
Motif MOTIF 734..736; /note=Cell attachment site; /evidence=ECO:0000255; MOTIF 1082..1084; /note=Cell attachment site; /evidence=ECO:0000255
Gene Encoded By
Mass 137,953
Kinetics
Metal Binding METAL 1266; /note=Calcium; /evidence=ECO:0000250; METAL 1268; /note=Calcium; /evidence=ECO:0000250; METAL 1269; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250; METAL 1271; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250; METAL 1274; /note=Calcium; /evidence=ECO:0000250
Rhea ID
Cross Reference Brenda