| IED ID | IndEnz0002004542 |
| Enzyme Type ID | protease004542 |
| Protein Name |
Calpain small subunit 1 CSS1 Calcium-activated neutral proteinase small subunit CANP small subunit Calcium-dependent protease small subunit CDPS Calcium-dependent protease small subunit 1 Calpain regulatory subunit |
| Gene Name | CAPNS1 CAPN4 |
| Organism | Bos taurus (Bovine) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Ruminantia Pecora Bovidae Bovinae Bos (oxen cattle) Bos taurus (Bovine) |
| Enzyme Sequence | MFLVNSFLKGGGGGGGGGGLGGGLGNVLGGLISGAGGGGGGGGGGGGGGGGTAMRILGGVISAISEAAAQYNPEPVPPRTHYSNIEANESEEVRQFRRLFAQLAGDDMEVSATELMNILNKVVTRHPDLKTDGFGIDTCRSMVAVMDSDTTGKLGFEEFKYLWNNIKKWQAVYKQFDVDRSGTIGSSELPGAFEAAGFRLNEHLYNMIIRRYSDEGGNMDFDNFISCLVRLDAMFRAFKSLDKDGTGQIQVNIQEWLQLTMYS |
| Enzyme Length | 263 |
| Uniprot Accession Number | P13135 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | CA_BIND 103..114; /note=1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; CA_BIND 147..158; /note=2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; CA_BIND 177..188; /note=3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448 |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Essential for embryonic development (By similarity). {ECO:0000250|UniProtKB:O88456}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Calcium binding (3); Chain (1); Domain (5); Metal binding (16); Modified residue (3) |
| Keywords | Acetylation;Calcium;Cell membrane;Cytoplasm;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Repeat |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Cell membrane {ECO:0000250}. Note=Translocates to the plasma membrane upon calcium binding. {ECO:0000250}. |
| Modified Residue | MOD_RES 1; /note=N-acetylmethionine; /evidence=ECO:0000250|UniProtKB:P04632; MOD_RES 6; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P04632; MOD_RES 174; /note=N6-acetyllysine; /evidence=ECO:0000250|UniProtKB:P04632 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 27,931 |
| Kinetics | |
| Metal Binding | METAL 104; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q64537; METAL 107; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:Q64537; METAL 109; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q64537; METAL 114; /note=Calcium 1; /evidence=ECO:0000250|UniProtKB:Q64537; METAL 132; /note=Calcium 4; /evidence=ECO:0000250|UniProtKB:Q64537; METAL 147; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:Q64537; METAL 149; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:Q64537; METAL 151; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q64537; METAL 153; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q64537; METAL 158; /note=Calcium 2; /evidence=ECO:0000250|UniProtKB:Q64537; METAL 177; /note=Calcium 3; /evidence=ECO:0000250|UniProtKB:Q64537; METAL 179; /note=Calcium 3; /evidence=ECO:0000250|UniProtKB:Q64537; METAL 181; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q64537; METAL 183; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:Q64537; METAL 188; /note=Calcium 3; /evidence=ECO:0000250|UniProtKB:Q64537; METAL 220; /note=Calcium 4; /evidence=ECO:0000250|UniProtKB:Q64537 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.22.B24; |