| IED ID | IndEnz0002004630 |
| Enzyme Type ID | protease004630 |
| Protein Name |
Germination protease EC 3.4.24.78 GPR endopeptidase Germination proteinase Spore protease |
| Gene Name | gpr CPF_2298 |
| Organism | Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Clostridia Eubacteriales Clostridiaceae Clostridium Clostridium perfringens Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) |
| Enzyme Sequence | MYNVRTDLAVESREIYKHRYNREIDGVVFEEKTVEEDIKVTNVDILNEEGAKAMGKPIGRYVTIDIPEYTHYDGGIMDEVSHVVAASLEELINLPEERTALVVGLGNWNVTPDAIGPKVVGKLMVTRHLKKVMPDIIDDSVRPVCAIAPGVLGITGIETGEIIKSLVEKINPDLVVCIDALASRKLERVARTIQISNTGISPGAGVGNHRMQINEESLGIPVIALGVPTVVDAATIANDAMDLVLDEMINQADAGKEFYNILNNIDKNEKGMMIKSLLDPYVGDLMVTPKEIDDIIESVSKIIANGINIALQPNMVLEDINKFLN |
| Enzyme Length | 325 |
| Uniprot Accession Number | Q0TNR9 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endopeptidase action with P4 Glu or Asp, P1 preferably Glu > Asp, P1' hydrophobic and P2' Ala.; EC=3.4.24.78; Evidence={ECO:0000255|HAMAP-Rule:MF_00626}; |
| DNA Binding | |
| EC Number | 3.4.24.78 |
| Enzyme Function | FUNCTION: Initiates the rapid degradation of small, acid-soluble proteins during spore germination. {ECO:0000255|HAMAP-Rule:MF_00626}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Propeptide (1) |
| Keywords | Hydrolase;Protease;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Autoproteolytically processed. The inactive tetrameric zymogen termed p46 autoprocesses to a smaller form termed p41, which is active only during spore germination. {ECO:0000255|HAMAP-Rule:MF_00626}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 35,548 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |