| IED ID | IndEnz0002004633 |
| Enzyme Type ID | protease004633 |
| Protein Name |
Germination protease EC 3.4.24.78 GPR endopeptidase Germination proteinase Spore protease |
| Gene Name | gpr BMQ_4571 |
| Organism | Priestia megaterium (strain ATCC 12872 / QMB1551) (Bacillus megaterium) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Priestia Priestia megaterium (Bacillus megaterium) Priestia megaterium (strain ATCC 12872 / QMB1551) (Bacillus megaterium) |
| Enzyme Sequence | MEKELDLSQYSVRTDLAVEAKDIALENQPKPNNQSEIKGVIVKEKEEQGVKISMVEITEEGAEAIGKKKGRYVTLESVGIREQDTEKQEAMEEVFAKELNFFIKSLNIPDDASCLVVGLGNLSVTPDALGPKAVDNLLITRHLFELQPESVQDGFRPVSAIVPGVMGMTGIETSDIIFGVVKKVNPDFIIAIDALAARSIERVNATIQISDSGIHPGSGVGNKRKEISYETLGIPVIAIGIPTVVDAVSITSDTIDFILKHFGREMKEQGKPSKSLLPSGMTFGEKKKLTEDDLPNEEQRQTYLGMIGTLPDEEKRRLIHEVLAPLGHNLMVTPKEVDMFIEDMANVVAGGLNAALHHEVDQENFGAYTH |
| Enzyme Length | 370 |
| Uniprot Accession Number | P22321 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endopeptidase action with P4 Glu or Asp, P1 preferably Glu > Asp, P1' hydrophobic and P2' Ala.; EC=3.4.24.78; |
| DNA Binding | |
| EC Number | 3.4.24.78 |
| Enzyme Function | FUNCTION: Initiates the rapid degradation of small, acid-soluble proteins during spore germination. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (14); Chain (1); Helix (13); Propeptide (1); Sequence conflict (1); Turn (2) |
| Keywords | 3D-structure;Direct protein sequencing;Hydrolase;Protease;Reference proteome;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Autoproteolytically processed. The inactive tetrameric zymogen termed p46 autoprocesses to a smaller form termed p41, which is active only during spore germination. |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 1C8B; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 40,497 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.78; |