| IED ID | IndEnz0002004647 |
| Enzyme Type ID | protease004647 |
| Protein Name |
Insulin-degrading enzyme EC 3.4.24.56 Insulin protease Insulinase Insulysin |
| Gene Name | Ide CG5517 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MTIAESSQKSATRKPDSMEPILRLNNIEKSLQDTRDYRGLQLENGLKVLLISDPNTDVSAAALSVQVGHMSDPTNLPGLAHFCEHMLFLGTEKYPHENGYTTYLSQSGGSSNAATYPLMTKYHFHVAPDKLDGALDRFAQFFIAPLFTPSATEREINAVNSEHEKNLPSDLWRIKQVNRHLAKPDHAYSKFGSGNKTTLSEIPKSKNIDVRDELLKFHKQWYSANIMCLAVIGKESLDELEGMVLEKFSEIENKNVKVPGWPRHPYAEERYGQKVKIVPIKDIRSLTISFTTDDLTQFYKSGPDNYLTHLIGHEGKGSILSELRRLGWCNDLMAGHQNTQNGFGFFDIVVDLTQEGLEHVDDIVKIVFQYLEMLRKEGPKKWIFDECVKLNEMRFRFKEKEQPENLVTHAVSSMQIFPLEEVLIAPYLSNEWRPDLIKGLLDELVPSKSRIVIVSQSFEPDCDLAEPYYKTKYGITRVAKDTVQSWENCELNENLKLALPNSFIPTNFDISDVPADAPKHPTIILDTPILRVWHKQDNQFNKPKACMTFDMSNPIAYLDPLNCNLNHMMVMLLKDQLNEYLYDAELASLKLSVMGKSCGIDFTIRGFSDKQVVLLEKLLDHLFDFSIDEKRFDILKEEYVRSLKNFKAEQPYQHSIYYLALLLTENAWANMELLDAMELVTYDRVLNFAKEFFQRLHTECFIFGNVTKQQATDIAGRVNTRLEATNASKLPILARQMLKKREYKLLAGDSYLFEKENEFHKSSCAQLYLQCGAQTDHTNIMVNLVSQVLSEPCYDCLRTKEQLGYIVFSGVRKVNGANGIRIIVQSAKHPSYVEDRIENFLQTYLQVIEDMPLDEFERHKEALAVKKLEKPKTIFQQFSQFYGEIAMQTYHFEREEAEVAILRKISKADFVDYFKKFIAKDGEERRVLSVHIVSQQTDENATSEAEPVEITNMERHKPISDIVTFKSCKELYPIALPFLDIKAKGARSKL |
| Enzyme Length | 990 |
| Uniprot Accession Number | P22817 |
| Absorption | |
| Active Site | ACT_SITE 84; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Degradation of insulin, glucagon and other polypeptides. No action on proteins.; EC=3.4.24.56; |
| DNA Binding | |
| EC Number | 3.4.24.56 |
| Enzyme Function | FUNCTION: Can cleave insulin and TGF-alpha. {ECO:0000269|PubMed:3139025}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 37 degrees Celsius. {ECO:0000269|PubMed:3139025}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7-8. {ECO:0000269|PubMed:3139025}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Erroneous initiation (3); Initiator methionine (1); Metal binding (3); Sequence conflict (6) |
| Keywords | Direct protein sequencing;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Zinc |
| Interact With | |
| Induction | INDUCTION: Inhibited by bacitracin and sulfhydryl-specific reagents. {ECO:0000269|PubMed:3139025}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10924473; 12865422; 15234342; 15834148; 16107220; 18245849; 18690061; 19520911; 20220848; 20371351; 20493190; 21074052; 21212741; 21917925; 22758915; 23071443; 23445342; 23827522; 24430872; 25096240; 25294943; 25312911; 25740935; 26472340; 26526100; 2659071; 26865094; 26870755; 27172210; 27444168; 27794539; 28633019; 29523653; 30378026; 30541713; 30652125; 31208963; 32019113; 32984330; 33143302; 7610476; 8099278; 9128138; |
| Motif | |
| Gene Encoded By | |
| Mass | 113,684 |
| Kinetics | |
| Metal Binding | METAL 81; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096; METAL 85; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096; METAL 162; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU10096 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.56; |