| IED ID | IndEnz0002004666 |
| Enzyme Type ID | protease004666 |
| Protein Name |
Kunitz trypsin inhibitor 4 AtKTI4 Kunitz trypsin inhibitor 1 AtKTI1 Trypsin protease inhibitor |
| Gene Name | KTI4 KTI1 TPI At1g73260 T18K17.7 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MTKTTKTMNPKFYLVLALTAVLASNAYGAVVDIDGNAMFHESYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKKANVTEVSSKTM |
| Enzyme Length | 215 |
| Uniprot Accession Number | Q8RXD5 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Exhibits Kunitz trypsin protease inhibitor activity (PubMed:19825555). Involved in modulating programmed cell death (PCD) in plant-pathogen interactions (PubMed:19825555). Can inhibit both serine proteases and cysteine proteases (PubMed:30042779). May be involved in the modulation of the proteases that participate in the hydrolysis of dietary proteins in the gut of spider mites (PubMed:30042779). {ECO:0000269|PubMed:19825555, ECO:0000269|PubMed:30042779}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (1); Chain (1); Disulfide bond (2); Erroneous initiation (1); Glycosylation (1); Sequence conflict (2); Signal peptide (1) |
| Keywords | Alternative splicing;Apoptosis;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Plant defense;Protease inhibitor;Reference proteome;Serine protease inhibitor;Signal;Thiol protease inhibitor |
| Interact With | |
| Induction | INDUCTION: Down-regulated after root-knot nematode infection (PubMed:16236154). Accumulates locally within 72 hours after P.brassicae butterflies oviposition (PubMed:17142483). Induced late in response to bacterial and fungal elicitors (e.g. Pst DC3000 and Ecc culture filtrates), and upon wounding, salicylic acid (SA) and hydrogen peroxide H(2)O(2) treatments (PubMed:19825555). Induced by infestation with spider mites (PubMed:30042779). {ECO:0000269|PubMed:16236154, ECO:0000269|PubMed:17142483, ECO:0000269|PubMed:19825555, ECO:0000269|PubMed:30042779}. |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum {ECO:0000269|PubMed:30042779}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..28; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11115874; 11739776; 14617064; 14671022; 14982972; 15144382; 15200641; 15272001; 15272873; 15282545; 15319476; 15516340; 16262716; 16623907; 17098848; 17888165; 17916636; 18539778; 18650403; 18783601; 18796151; 23858430; 28627464; 32796124; |
| Motif | |
| Gene Encoded By | |
| Mass | 23,793 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |