| IED ID | IndEnz0002004669 |
| Enzyme Type ID | protease004669 |
| Protein Name |
Lon protease homolog 2, peroxisomal EC 3.4.21.53 |
| Gene Name | AFUA_5G11750 |
| Organism | Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Fumigati Neosartorya fumigata (Aspergillus fumigatus) Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) |
| Enzyme Sequence | MGSNNGRAMKLALVPLPKGSVLLPGVTLRIPVSNRPDLANLLSALLDQTNLGKRDGNTITFGCVPLRSPLLSNDGQQLIDDGSVDGAKKEEFDAIDAGQARKEDLFRYGTVGKVIGVQRRAYAEPFLVVQGVQRFTIKHILRERPFFEGEVVLHNERDAISSDAETVELFQQLRQLSRELITLLRLSSLLPSTGTRLSPLVARKFEVYIAKTDLSQAGNLADFMADVADPTFEEKLRVLASFALRTRLERVVELLARQVQGIKNSVKVTTISTSSFPSNSPFDISQIDPRDRELLARRVMAGLTGLTPPGAAGGRNNEDEKETNEVDELQKRLQEAELSPEARKVADKELRRLRKMNPANAEYGVCRTYLENIADIPWTKVTEDKLGPETLKRARNQLDEDHYGLETIKKRLLEYLAVLRLKQSTNQDVERQIAALTKELDAANEVLAEKDVPALSESDRVSLEAKLNLLQSRRLADKSPILLLVGPPGTGKTSLARSVATSLGRKFHRISLGGVRDEAEIRGHRRTYVAAMPGLIVNGLKKVGVANPVFLLDEIDKVGGANFQGDPSAAMLEVLDPEQNSTFVDHYINIPIDLSKVLFIATANSLDTIPAPLLDRMETITLSGYTTVEKRHIAKRHLIPKQIRANGLAEGQVVLSDEVVDKVITSYTRESGVRNLERELGSICRHKAVQYADAVDNGRLDTYNPVVALGDLEDILGIERFDEEIAEKHGRPGVVTGLVAYSTGGQGSILFIEVADMPGNGRVQLTGKLGDVLKESVEVALTWVKAHSFELGLTHDPNEDIMKNRSLHVHCPAGAIPKDGPSAGLAHTIGLISLFTGKAVPPQIAMTGEVSLRGRVMPVGGIKEKLIGAHRAGVKTVLLPEQNRKDVKDVPQEVHDGLQIVYVRHIWEAIRQVWPGAHWPGQHHINFVESRL |
| Enzyme Length | 932 |
| Uniprot Accession Number | Q4WVD9 |
| Absorption | |
| Active Site | ACT_SITE 822; /evidence=ECO:0000255|HAMAP-Rule:MF_03121; ACT_SITE 865; /evidence=ECO:0000255|HAMAP-Rule:MF_03121 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins in presence of ATP.; EC=3.4.21.53; Evidence={ECO:0000255|HAMAP-Rule:MF_03121}; |
| DNA Binding | |
| EC Number | 3.4.21.53 |
| Enzyme Function | FUNCTION: ATP-dependent serine protease that mediates the selective degradation of misfolded and unassembled polypeptides in the peroxisomal matrix. Necessary for type 2 peroxisome targeting signal (PTS2)-containing protein processing and facilitates peroxisome matrix protein import. {ECO:0000255|HAMAP-Rule:MF_03121}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 486..493; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_03121 |
| Features | Active site (2); Chain (1); Compositional bias (1); Domain (2); Motif (1); Nucleotide binding (1); Region (1) |
| Keywords | ATP-binding;Hydrolase;Nucleotide-binding;Peroxisome;Protease;Reference proteome;Serine protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Peroxisome matrix {ECO:0000255|HAMAP-Rule:MF_03121}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 930..932; /note=Microbody targeting signal; /evidence=ECO:0000255|HAMAP-Rule:MF_03121 |
| Gene Encoded By | |
| Mass | 102,474 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |