| IED ID | IndEnz0002004706 |
| Enzyme Type ID | protease004706 |
| Protein Name |
E3 ubiquitin-protein ligase BOI EC 2.3.2.27 Inhibitor of apoptosis IAP -like protein AtILP Protein BOTRYTIS SUSCEPTIBLE 1 INTERACTOR AtBOI RING-type E3 ubiquitin transferase BOI |
| Gene Name | BOI ILP At4g19700 T16H5.60 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAVQAHHMNIFSQFISPNRDCVKFQENMNHGEFEFTGGEVPLITGESFAVEPLAAKANFNKAESGLSYNFTVPPLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPSLINVELVSQIQNQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKDDEIVRIRNLNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQLETFPTASAVVEDDAESSCGSCCGDGGGEAVTAVGGGCKRCGEREASVLVLPCRHLCLCTVCGGSALLRTCPVCDMVMNASVHVNMSS |
| Enzyme Length | 304 |
| Uniprot Accession Number | O81851 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; Evidence={ECO:0000269|PubMed:20921156}; |
| DNA Binding | |
| EC Number | 2.3.2.27 |
| Enzyme Function | FUNCTION: E3 ubiquitin-protein ligase involved in the regulation of pathogen and abiotic stress responses by facilitating degradation of MYB108/BOI. Attenuates cell death by preventing caspase activation. Has no effect on the stability of the DELLA proteins. Not regulated by MYB108/BOI. {ECO:0000269|PubMed:20921156, ECO:0000269|PubMed:21926169, ECO:0000269|PubMed:23482857}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Protein degradation; proteasomal ubiquitin-dependent pathway. |
| nucleotide Binding | |
| Features | Chain (1); Coiled coil (1); Region (1); Sequence conflict (2); Zinc finger (1) |
| Keywords | Apoptosis;Coiled coil;Metal-binding;Nucleus;Plant defense;Reference proteome;Transferase;Ubl conjugation pathway;Zinc;Zinc-finger |
| Interact With | |
| Induction | INDUCTION: Up-regulated by pathogen, methyl violagen, salicylic acid, 1-aminocyclopropane-1-carboxylic acid (ACC) and salt. Down-regulated by methyl jasmonate and gibberellic acid. {ECO:0000269|PubMed:20921156}. |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20921156, ECO:0000269|PubMed:21926169}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11983057; 17933900; 19944772; 20195657; 20736450; 21798944; 26298008; 28017649; 30169501; |
| Motif | |
| Gene Encoded By | |
| Mass | 33,509 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |