| IED ID | IndEnz0002004710 |
| Enzyme Type ID | protease004710 |
| Protein Name |
Carbohydrate-binding protein AWN Sperm-associated protein AWN Spermadhesin AWN Zona pellucida-binding protein AWN |
| Gene Name | |
| Organism | Sus scrofa (Pig) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Suina Suidae (pigs) Sus Sus scrofa (Pig) |
| Enzyme Sequence | MKLAAPSLALLLSTATLVSGAWNRRSRSCGGVLRDPPGKIFNSDGPQKDCVWTIKVKPHFHVVLAIPPLNLSCGKEYVELLDGPPGSEIIGKICGGISLVFRSSSNIATIKYLRTSGQRASPFHIYYYADPEGPLQFPYFDRQTIIATEKNIP |
| Enzyme Length | 153 |
| Uniprot Accession Number | P26776 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: AWN proteins mediate the binding of boar spermatozoa to component(s) of the egg's zona pellucida by a carbohydrate-binding mechanism. Awn proteins are secretory components of the male accessory glands being coated to the sperm surface at the time of ejaculation. They possess as well heparin-, serine-protease-inhibitor-binding capability. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Disulfide bond (2); Domain (1); Modified residue (1); Region (1); Sequence conflict (4); Signal peptide (1) |
| Keywords | Acetylation;Direct protein sequencing;Disulfide bond;Fertilization;Heparin-binding;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. Note=Predominantly localized on the membrane overlying the acrosomal cap region of the sperm head. |
| Modified Residue | MOD_RES 21; /note=N-acetylalanine; /evidence=ECO:0000269|PubMed:1555646 |
| Post Translational Modification | PTM: Partial N-acetylation differentiates isoforms AWN-1 (not acetylated) and AWN-2 (acetylated). |
| Signal Peptide | SIGNAL 1..20; /evidence="ECO:0000269|PubMed:1555646, ECO:0000269|PubMed:8603690" |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 16,730 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |