| IED ID | IndEnz0002004716 |
| Enzyme Type ID | protease004716 |
| Protein Name |
COP9 signalosome complex subunit 4 SGN4 Signalosome subunit 4 JAB1-containing signalosome subunit 4 |
| Gene Name | COPS4 CSN4 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MAAAVRQDLAQLMNSSGSHKDLAGKYRQILEKAIQLSGAEQLEALKAFVEAMVNENVSLVISRQLLTDFCTHLPNLPDSTAKEIYHFTLEKIQPRVISFEEQVASIRQHLASIYEKEEDWRNAAQVLVGIPLETGQKQYNVDYKLETYLKIARLYLEDDDPVQAEAYINRASLLQNESTNEQLQIHYKVCYARVLDYRRKFIEAAQRYNELSYKTIVHESERLEALKHALHCTILASAGQQRSRMLATLFKDERCQQLAAYGILEKMYLDRIIRGNQLQEFAAMLMPHQKATTADGSSILDRAVIEHNLLSASKLYNNITFEELGALLEIPAAKAEKIASQMITEGRMNGFIDQIDGIVHFETREALPTWDKQIQSLCFQVNNLLEKISQTAPEWTAQAMEAQMAQ |
| Enzyme Length | 406 |
| Uniprot Accession Number | Q9BT78 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. Also involved in the deneddylation of non-cullin subunits such as STON2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1, IRF8/ICSBP and SNAPIN, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. {ECO:0000269|PubMed:11285227, ECO:0000269|PubMed:11337588, ECO:0000269|PubMed:12628923, ECO:0000269|PubMed:12732143, ECO:0000269|PubMed:21102408, ECO:0000269|PubMed:9535219}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (1); Beta strand (4); Chain (1); Domain (1); Helix (24); Initiator methionine (1); Modified residue (2); Sequence conflict (2); Turn (2) |
| Keywords | 3D-structure;Acetylation;Alternative splicing;Cell junction;Cytoplasm;Cytoplasmic vesicle;Direct protein sequencing;Nucleus;Reference proteome;Signalosome;Synapse |
| Interact With | Q0VDD7; Q7L5N1; P01100; P08727; Q9BVL2; Q9Y5F1; O75381; Q15831; Q8N6Y0 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:9535219}. Nucleus {ECO:0000269|PubMed:9535219}. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle {ECO:0000269|PubMed:21102408}. |
| Modified Residue | MOD_RES 2; /note="N-acetylalanine"; /evidence="ECO:0000269|PubMed:18850735, ECO:0007744|PubMed:19413330"; MOD_RES 25; /note="N6-acetyllysine"; /evidence="ECO:0007744|PubMed:19608861" |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | Electron microscopy (5); X-ray crystallography (4) |
| Cross Reference PDB | 4D0P; 4D10; 4D18; 4WSN; 6R6H; 6R7F; 6R7H; 6R7I; 6R7N; |
| Mapped Pubmed ID | 10391953; 12944386; 14742321; 15161933; 15811629; 15882621; 16189514; 16223728; 16401342; 16916636; 16943200; 17183367; 17353931; 17882165; 18093314; 18835386; 18926707; 19109893; 19214193; 19367725; 19609301; 19615732; 19748355; 20195357; 20360068; 20855601; 21135142; 21388382; 21903422; 22118460; 22190034; 22653443; 22959436; 23238014; 23453757; 23535662; 23535663; 23583660; 24725084; 25043011; 25416956; 25609649; 25852190; 26496610; 26638075; 27029275; 30896450; 30992253; 31431478; 31444342; 31871319; |
| Motif | |
| Gene Encoded By | |
| Mass | 46,269 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |