| IED ID | IndEnz0002004763 |
| Enzyme Type ID | protease004763 |
| Protein Name |
Capsid protein Core antigen Core protein HBcAg p21.5 |
| Gene Name | C |
| Organism | Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) (HBV-A) |
| Taxonomic Lineage | Viruses Riboviria Pararnavirae Artverviricota Revtraviricetes Blubervirales Hepadnaviridae Orthohepadnavirus (mammalian hepatitis B-type viruses) Hepatitis B virus (HBV) Hepatitis B virus subtype adw (HBV) Hepatitis B virus subtype adw2 Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) (HBV-A) |
| Enzyme Sequence | MDIDPYKEFGATVELLSFLPSDFFPSVRDLLDTASALYREALESPEHCSPHHTALRQAILCWGELMTLATWVGNNLEDPASRDLVVNYVNTNVGLKIRQLLWFHISCLTFGRETVLEYLVSFGVWIRTPPAYRPPNAPILSTLPETTVVRRRDRGRSPRRRTPSPRRRRSPSPRRRRSQSRESQC |
| Enzyme Length | 185 |
| Uniprot Accession Number | P03148 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Self assembles to form an icosahedral capsid. Most capsids appear to be large particles with an icosahedral symmetry of T=4 and consist of 240 copies of capsid protein, though a fraction forms smaller T=3 particles consisting of 180 capsid proteins. Entering capsids are transported along microtubules to the nucleus. Phosphorylation of the capsid is thought to induce exposure of nuclear localization signal in the C-terminal portion of the capsid protein that allows binding to the nuclear pore complex via the importin (karyopherin-) alpha and beta. Capsids are imported in intact form through the nuclear pore into the nuclear basket, where it probably binds NUP153. Only capsids that contain the mature viral genome can release the viral DNA and capsid protein into the nucleoplasm. Immature capsids get stuck in the basket. Capsids encapsulate the pre-genomic RNA and the P protein. Pre-genomic RNA is reverse-transcribed into DNA while the capsid is still in the cytoplasm. The capsid can then either be directed to the nucleus, providing more genomes for transcription, or bud through the endoplasmic reticulum to provide new virions. {ECO:0000255|HAMAP-Rule:MF_04076}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (1); Modified residue (3); Motif (1); Mutagenesis (7); Natural variant (3); Region (3); Repeat (3) |
| Keywords | Alternative initiation;Capsid protein;Cytoplasmic inwards viral transport;DNA-binding;Host cytoplasm;Host-virus interaction;Microtubular inwards viral transport;Phosphoprotein;RNA-binding;Repeat;T=4 icosahedral capsid protein;Viral penetration into host nucleus;Virion;Virus entry into host cell |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04076, ECO:0000269|PubMed:3006057}. Host cytoplasm {ECO:0000255|HAMAP-Rule:MF_04076, ECO:0000269|PubMed:3006057}. |
| Modified Residue | MOD_RES 157; /note="Phosphoserine; by host"; /evidence="ECO:0000255|HAMAP-Rule:MF_04076, ECO:0000269|PubMed:7815479"; MOD_RES 164; /note="Phosphoserine; by host"; /evidence="ECO:0000255|HAMAP-Rule:MF_04076, ECO:0000269|PubMed:7815479"; MOD_RES 172; /note="Phosphoserine; by host"; /evidence="ECO:0000255|HAMAP-Rule:MF_04076, ECO:0000269|PubMed:7815479" |
| Post Translational Modification | PTM: Phosphorylated by host SRPK1, SRPK2, and maybe protein kinase C or GAPDH. Phosphorylation is critical for pregenomic RNA packaging. Protein kinase C phosphorylation is stimulated by HBx protein and may play a role in transport of the viral genome to the nucleus at the late step during the viral replication cycle. {ECO:0000255|HAMAP-Rule:MF_04076, ECO:0000269|PubMed:7815479, ECO:0000269|PubMed:9680129}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 160..177; /note=Bipartite nuclear localization signal; /evidence=ECO:0000255|HAMAP-Rule:MF_04076 |
| Gene Encoded By | |
| Mass | 21,332 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |