| IED ID | IndEnz0002004766 |
| Enzyme Type ID | protease004766 |
| Protein Name |
Calpain-type cysteine protease DEK1 EC 3.4.22.- Phytocalpain DEK1 Protein DEFECTIVE KERNEL 1 AtDEK1 Protein EMBRYO DEFECTIVE 1275 Protein EMBRYO DEFECTIVE 80 |
| Gene Name | DEK1 EMB1275 EMB80 At1g55350 F7A10.23 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MEGDERGVLLACVISGTLFTVFGSGSFWILWAVNWRPWRLYSWIFARKWPKVLQGPQLDILCGVLSLFAWIVVVSPIAILIGWGSWLIVILDRHIIGLAIIMAGTALLLAFYSIMLWWRTQWQSSRAVALLLLLGVALLCAYELCAVYVTAGAHASQQYSPSGFFFGVSAIALAINMLFICRMVFNGNGLDVDEYVRRAYKFAYSDCIEVGPVACLPEPPDPNELYPRQTSRASHLGLLYLGSLVVLLAYSVLYGLTARESRWLGGITSAAVIVLDWNIGACLYGFKLLQNRVLALFVAGISRLFLICFGIHYWYLGHCISYIFVASVLSGAAVSRHLSITDPSAARRDALQSTVIRLREGFRRKEQNSSSSSSDGCGSSIKRSSSIDAGHTGCTNEANRTAESCTADNLTRTGSSQEGINSDKSEESGRPSLGLRSSSCRSVVQEPEAGTSYFMDKVSDQNNTLVVCSSSGLDSQGYESSTSNSANQQLLDMNLALAFQDQLNNPRIASILKKKAKEGDLELTNLLQDKGLDPNFAVMLKEKNLDPTILALLQRSSLDADRDHRDNTDITIIDSNSVDNTLPNQISLSEELRLRGLEKWLKLSRLLLHHVAGTPERAWGLFSLVFILETIIVAIFRPKTITIINSSHQQFEFGFSVLLLSPVVCSIMAFLRSLQVEEMALTSKSRKYGFVAWLLSTSVGLSLSFLSKSSVLLGISLTVPLMAACLSIAVPIWMHNGYQFWVPQLSCGDQARDLRSPRIKGFILWICVVLFAGSVISLGAIISAKPLDDLKYKLFSARENNVTSPYTSSVYLGWAMSSGIALVVTAILPIVSWFATYRFSHSSAVCLMIFSVVLVAFCGTSYLEVVKSRDDQLPTKGDFLAALLPLACIPALLSLCCGMVKWKDDCWILSRGVYVFFSIGLLLLFGAIAAVIAVKPWTIGVSFLLVLFLMVVTIGVIHLWASNNFYLTRKQTSFVCFLALLLGLAAFLLGWHQDKAFAGASVGYFTFLSLLAGRALAVLLSPPIVVYSPRVLPVYVYDAHADCGKNVSAAFLVLYGIALATEGWGVVASLIIYPPFAGAAVSAITLVVAFGFAVSRPCLTLEMMEVAVRFLSKDTIVQAISRSATKTRNALSGTYSAPQRSASSAALLVGDPSAMRDKAGNFVLPRDDVMKLRDRLRNEERVAGSIFYKMQCRKGFRHEPPTNVDYRRDMCAHARVLALEEAIDTEWVYMWDKFGGYLLLLLGLTAKAERVQDEVRLRLFLDSIGFSDLSARKISKWKPEDRRQFEIIQESYLREKEMEEESLMQRREEEGRGKERRKALLEKEERKWKEIEASLIPSIPNAGSREAAAMAAAIRAVGGDSVLEDSFARERVSGIARRIRTAQLERRAQQTGISGAVCVLDDEPMISGKHCGQMDSSVCQSQKISFSVTAMIQSDSGPVCLFGTEFQKKVCWEILVAGSEQGIEAGQVGLRLITKGERQTTVAREWYIGATSITDGRWHTVTITIDADAGEATCYIDGGFDGYQNGLPLSIGSAIWEQGAEVWLGVRPPIDVDAFGRSDSDGVESKMHIMDVFLWGKCLSEEEAASLHAAIGMADLDMIDLSDDNWQWTDSPPRVDGWDSDPADVDLYDRDDVDWDGQYSSGRKRRSGRDFVMSVDSFARRHRKPRMETQEDINQRMRSVELAVKEALSARGDKQFTDQEFPPNDRSLFVDTQNPPSKLQVVSEWMRPDSIVKENGSDSRPCLFSGDANPSDVCQGRLGDCWFLSAVAVLTEVSRISEVIITPEYNEEGIYTVRFCIQGEWVPVVIDDWIPCESPGKPAFATSRKLNELWVSMVEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEEIDLRSAQAQIDLASGRLWSQLLRFKQEGFLLGAGSPSGSDVHVSSSGIVQGHAYSVLQVREVDGHRLVQIRNPWANEVEWNGPWSDSSPEWTDRMKHKLKHVPQSKEGIFWMSWQDFQIHFRSIYVCRVYPREMRYSVNGQWRGYSAGGCQDYSSWHQNPQFRLRATGSDASLPIHVFITLTQGVGFSRTTPGFRNYQSSHDSQLFYIGLRILKTRGRRAAYNIFLHESVGGTDYVNSREISCEMVLDPDPKGYTIVPTTIHPGEEAPFVLSVFTKASIVLEAL |
| Enzyme Length | 2151 |
| Uniprot Accession Number | Q8RVL2 |
| Absorption | |
| Active Site | ACT_SITE 1761; /evidence=ECO:0000250; ACT_SITE 1919; /evidence=ECO:0000250; ACT_SITE 1939; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Essential protease involved in epiderm development. Required for aleurone cell development in the endosperm probably by maintaining and restricting the aleurone and embryonic epidermal L1 cell-layer fates as well as meristems organization. Involved in the maintenance of adaxial/abaxial axis information in developing leaves, probably by regulating cell proliferation and expansion. Does not need calcium ions to be active. Required for the formation of giant cells in sepals by determining cell fate and promoting endoreplication. {ECO:0000269|PubMed:15647902, ECO:0000269|PubMed:16167900, ECO:0000269|PubMed:17933905, ECO:0000269|PubMed:18952779, ECO:0000269|PubMed:23095885}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Compositional bias (1); Domain (2); Erroneous gene model prediction (1); Mutagenesis (2); Propeptide (1); Region (2); Signal peptide (1); Topological domain (22); Transmembrane (21) |
| Keywords | Alternative splicing;Cell membrane;Cytoplasm;Developmental protein;Endoplasmic reticulum;Endosome;Hydrolase;Membrane;Protease;Reference proteome;Repeat;Signal;Thiol protease;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cytoplasm. |
| Modified Residue | |
| Post Translational Modification | PTM: Autocatalytic proteolytic cleavage leading to the production of mainly cytoplasmic localized subproducts of about 85 and 120 kDa. |
| Signal Peptide | SIGNAL 1..32; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 18642946; 23663131; 25953348; 26220906; 27756823; 28692330; 29044106; 29782638; 30617799; |
| Motif | |
| Gene Encoded By | |
| Mass | 238,264 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |