| IED ID | IndEnz0002004854 |
| Enzyme Type ID | protease004854 |
| Protein Name |
NEDD8-specific protease 1 EC 3.4.22.- |
| Gene Name | nep1 SPBC17D11.01 SPBC20F10.11 |
| Organism | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota Taphrinomycotina Schizosaccharomycetes Schizosaccharomycetales Schizosaccharomycetaceae Schizosaccharomyces Schizosaccharomyces pombe (Fission yeast) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Enzyme Sequence | MSSSPTVLELFDVCFKQEDVDSLKKPNWFTDVSIDYVDELIEHLWFPSYPNQANEILLLRPSLVFLLAEAAISPEELKVALPKKLMNCKYLFMPINDLDKHAAGSGGSHWSLMVASIPDGQCYYYDSLSNGKTKDCRSALARVSDLFKKKFTIECMPVQQQRNGYDCGAHVCAFTLELVRRLLHSPMPTSSMWNLSTFQPDVTAIREQLSRCLDHIINSLGTRVSGDFDEDFPTGTVFFDLESHLPLLDVALPVLPKSSDSSETSHESSNSNLKKSSESGSTNHHNNHESDKDLHHEGHHHHHHHHHHHHSHDDDPSSPAEKKQNHVPSPSEKIQDHVPSPSEKKQDRVPSPSNNKEDHLPLLSDEKLDKSAIDKIEPTPLPSVHMNSHIAKGELPKFHNSTDNPFLTPPEELVSGDFPF |
| Enzyme Length | 420 |
| Uniprot Accession Number | O42980 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as the pcu1, pcu2 and pcu4 cullins and other proteins. {ECO:0000269|PubMed:15769255}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (4); Modified residue (3); Region (1); Sequence conflict (2) |
| Keywords | Cytoplasm;Hydrolase;Phosphoprotein;Protease;Reference proteome;Thiol protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15769255}. |
| Modified Residue | MOD_RES 329; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18257517; MOD_RES 340; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18257517; MOD_RES 351; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:18257517 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 19547744; 20473289; 21712547; 23697806; 24763107; 25720772; 26404184; 26644575; 29996109; 30647105; 30726745; |
| Motif | |
| Gene Encoded By | |
| Mass | 47,258 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |