| IED ID | IndEnz0002004866 |
| Enzyme Type ID | protease004866 |
| Protein Name |
Lon-like protease BrxL BREX protein BrxL |
| Gene Name | brxL BCH308197_0968 |
| Organism | Bacillus cereus (strain H3081.97) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus cereus group Bacillus cereus Bacillus cereus (strain H3081.97) |
| Enzyme Sequence | MEDLNIKLNAHFAGKVVRKDLTKKIKEGANVPVYVLEYLLGMYCATDDEKSMNDGVQMVKKILSDNFVRPDEAEKVKSKVKELGKYTVIDKIGVKLNDKKDIYEAEFSNLGLNGVPISSHYVKEFDKLLAGGIWCIVKMEYYFDEESKGTSPFSIESVTPIQMPNMDLEEMFEQRRQFSKEEWIDVLIRSTGMEPTQLEDTVKWHLLERMVPLVENNYNLCELGPRGTGKSHIYKEISPNSILVSGGQTTVANLFYNMSTRKIGLVGMWDTVAFDEVAGITFKDKDGIQIMKDYMASGSFARGREEKNASASMVFVGNINQSVDVLLKTSHLFDPFPEAMAYDSAFFDRMHYYLPGWEIPKMRPEFFTNEYGFITDYLAEFLREMRKRSFSDAIDKYFRLGNNLNQRDVIAVRKTVSGLIKLLYPNGEYIKEDVEEVLRYALIGRRRVKEQLKKIGGMEFYDVNFSYIDNESMNEEFVSVPEQGGGTLIPEGMNKPGHIYTVARGKTGMIGTYKLETEVVSGNGKFEKTGLNSDRDAKESIDTAFRFFKANNKNISGTISTTTKDYLMHIQDIHGVGLTGELSLAAFIALCSGALNKPVQSQMVVLGSISISGTINKVEELANVLQVCFDSGAKKILLPMVSAVDIPTVPPELFAKFQIGFYQSAEDAVFKALGVE |
| Enzyme Length | 676 |
| Uniprot Accession Number | P0DUF5 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: BREX systems (bacteriophage exclusion) provide immunity against bacteriophage. Part of a type 1 BREX system. This system allows phage adsorption but prevents phage DNA replication, without degradation of the phage DNA. Methylation of bacterial DNA by PglX probably guides self/non-self discrimination. When the brxA-brxB-brxC-pglX and pglZ-brxL operons are transformed into a susceptible B.subtilis strain (BEST7003) they confer resistance to bacteriophages SPbeta, SP16, Zeta, phi3T and SP02 and partial protection to phages SP01 and SP82G (these include lytic and temperate phage). They do not protect against phages phi105, rho10 or rho14. Additionally confers a very slight reduction in efficiency of plasmid transformation. {ECO:0000269|PubMed:25452498}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1) |
| Keywords | Antiviral defense;Hydrolase;Protease |
| Interact With | |
| Induction | INDUCTION: Part of the pglZ-brxL operon. {ECO:0000269|PubMed:25452498}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 76,095 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |