| IED ID | IndEnz0002004880 |
| Enzyme Type ID | protease004880 |
| Protein Name |
Cathepsin S EC 3.4.22.27 |
| Gene Name | CTSS |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MKRLVCVLLVCSSAVAQLHKDPTLDHHWHLWKKTYGKQYKEKNEEAVRRLIWEKNLKFVMLHNLEHSMGMHSYDLGMNHLGDMTSEEVMSLMSSLRVPSQWQRNITYKSNPNRILPDSVDWREKGCVTEVKYQGSCGACWAFSAVGALEAQLKLKTGKLVSLSAQNLVDCSTEKYGNKGCNGGFMTTAFQYIIDNKGIDSDASYPYKAMDQKCQYDSKYRAATCSKYTELPYGREDVLKEAVANKGPVSVGVDARHPSFFLYRSGVYYEPSCTQNVNHGVLVVGYGDLNGKEYWLVKNSWGHNFGEEGYIRMARNKGNHCGIASFPSYPEI |
| Enzyme Length | 331 |
| Uniprot Accession Number | P25774 |
| Absorption | |
| Active Site | ACT_SITE 139; /evidence=ECO:0000250; ACT_SITE 278; /evidence=ECO:0000250; ACT_SITE 298; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Similar to cathepsin L, but with much less activity on Z-Phe-Arg-|-NHMec, and more activity on the Z-Val-Val-Arg-|-Xaa compound.; EC=3.4.22.27; |
| DNA Binding | |
| EC Number | 3.4.22.27 |
| Enzyme Function | FUNCTION: Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules and MHC class II antigen presentation (PubMed:30612035). The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L. {ECO:0000269|PubMed:30612035}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (1); Beta strand (12); Chain (1); Disulfide bond (4); Glycosylation (1); Helix (16); Mutagenesis (1); Natural variant (2); Propeptide (1); Sequence conflict (2); Signal peptide (1); Turn (2) |
| Keywords | 3D-structure;Alternative splicing;Cytoplasmic vesicle;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Protease;Reference proteome;Secreted;Signal;Thiol protease;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Lysosome {ECO:0000269|PubMed:1377692}. Secreted {ECO:0000269|PubMed:9524075}. Cytoplasmic vesicle, phagosome {ECO:0000269|PubMed:30612035}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (59) |
| Cross Reference PDB | 1GLO; 1MS6; 1NPZ; 1NQC; 2C0Y; 2F1G; 2FQ9; 2FRA; 2FRQ; 2FT2; 2FUD; 2FYE; 2G6D; 2G7Y; 2H7J; 2HH5; 2HHN; 2HXZ; 2OP3; 2R9M; 2R9N; 2R9O; 3IEJ; 3N3G; 3N4C; 3OVX; 4P6E; 4P6G; 5QBU; 5QBV; 5QBW; 5QBX; 5QBY; 5QBZ; 5QC0; 5QC1; 5QC2; 5QC3; 5QC4; 5QC5; 5QC6; 5QC7; 5QC8; 5QC9; 5QCA; 5QCB; 5QCC; 5QCD; 5QCE; 5QCF; 5QCG; 5QCH; 5QCI; 5QCJ; 6YYN; 6YYO; 6YYP; 6YYQ; 6YYR; |
| Mapped Pubmed ID | 10072072; 10631941; 10748235; 10809722; 10809954; 10966814; 11684289; 11813165; 11853874; 12078484; 12164923; 12437117; 12459015; 12641451; 12643447; 12742663; 12748383; 12819022; 14966190; 15308097; 16140306; 16181339; 16338279; 16354158; 16394095; 16410454; 16446091; 16550604; 16825321; 16839849; 16876402; 17034136; 17075137; 17227755; 17601350; 17622942; 17878156; 18166152; 18410260; 18565099; 18572095; 18617639; 18820679; 18931679; 18957408; 18977241; 19101991; 19104823; 19404922; 19487544; 19553543; 19593952; 19700239; 19773165; 19849712; 19913121; 20045715; 20164293; 20180636; 20188543; 20356901; 20541404; 20580231; 20598883; 20628086; 20711500; 20880494; 20881085; 21030256; 21038029; 21094118; 21112096; 21143385; 21223957; 21250859; 21315176; 21402738; 21585286; 21604257; 21756348; 21878432; 21896479; 21967108; 21989182; 22101325; 22365146; 22424724; 22571763; 22730330; 22761382; 22796222; 22844403; 22844527; 22923671; 22952693; 23152404; 23362198; 23439581; 23650620; 23707200; 23742758; 23747398; 23871919; 23892358; 24335416; 24390241; 24523067; 24580730; 24583396; 24690038; 24727728; 24875536; 24940638; 24964046; 25086747; 25118282; 25313327; 25626674; 26029922; 26079659; 26081835; 26960148; 27058412; 27249607; 27372424; 27387133; 27595325; 27771373; 28236030; 28240259; 28289191; 28981104; 29181829; 29362520; 29624112; 29749483; 29915952; 29976774; 30006610; 30185799; 30261474; 30341237; 30423938; 30549421; 30641028; 30848029; 31017016; 31137114; 31319889; 31405651; 31605630; 32007579; 32307906; 32476947; 32515028; 32657442; 32887380; 33062070; 33148388; 33746900; 33754020; 7547873; 8626565; 8687433; 9008168; 9065415; 9115292; 9539769; 9545226; 9655332; |
| Motif | |
| Gene Encoded By | |
| Mass | 37,496 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.22.27; |