| IED ID | IndEnz0002004889 |
| Enzyme Type ID | protease004889 |
| Protein Name |
Caspase-10 CASP-10 EC 3.4.22.63 Apoptotic protease Mch-4 FAS-associated death domain protein interleukin-1B-converting enzyme 2 FLICE2 ICE-like apoptotic protease 4 Cleaved into: Caspase-10 subunit p23/17; Caspase-10 subunit p12 |
| Gene Name | CASP10 MCH4 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MKSQGQHWYSSSDKNCKVSFREKLLIIDSNLGVQDVENLKFLCIGLVPNKKLEKSSSASDVFEHLLAEDLLSEEDPFFLAELLYIIRQKKLLQHLNCTKEEVERLLPTRQRVSLFRNLLYELSEGIDSENLKDMIFLLKDSLPKTEMTSLSFLAFLEKQGKIDEDNLTCLEDLCKTVVPKLLRNIEKYKREKAIQIVTPPVDKEAESYQGEEELVSQTDVKTFLEALPQESWQNKHAGSNGNRATNGAPSLVSRGMQGASANTLNSETSTKRAAVYRMNRNHRGLCVIVNNHSFTSLKDRQGTHKDAEILSHVFQWLGFTVHIHNNVTKVEMEMVLQKQKCNPAHADGDCFVFCILTHGRFGAVYSSDEALIPIREIMSHFTALQCPRLAEKPKLFFIQACQGEEIQPSVSIEADALNPEQAPTSLQDSIPAEADFLLGLATVPGYVSFRHVEEGSWYIQSLCNHLKKLVPRMLKFLEKTMEIRGRKRTVWGAKQISATSLPTAISAQTPRPPMRRWSSVS |
| Enzyme Length | 521 |
| Uniprot Accession Number | Q92851 |
| Absorption | |
| Active Site | ACT_SITE 358; /evidence=ECO:0000250; ACT_SITE 401; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Leu-Gln-Thr-Asp-|-Gly.; EC=3.4.22.63; |
| DNA Binding | |
| EC Number | 3.4.22.63 |
| Enzyme Function | FUNCTION: Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. {ECO:0000269|PubMed:11717445}.; FUNCTION: Isoform 7 can enhance NF-kappaB activity but promotes only slight apoptosis. {ECO:0000269|PubMed:17822854}.; FUNCTION: Isoform C is proteolytically inactive. {ECO:0000269|PubMed:11717445}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (7); Chain (2); Domain (2); Mutagenesis (1); Natural variant (14); Propeptide (1); Region (1); Sequence conflict (2) |
| Keywords | Alternative splicing;Apoptosis;Direct protein sequencing;Disease variant;Hydrolase;Protease;Reference proteome;Repeat;Thiol protease;Zymogen |
| Interact With | Q14790; O15519; O14730; Q13546; P98170; O15519; P98170; O14730 |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Cleavage by granzyme B and autocatalytic activity generate the two active subunits. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11002417; 12037669; 12107169; 12198154; 12388546; 12884866; 12887920; 14687710; 14688482; 14761933; 15452117; 15767684; 16186808; 16251207; 16498403; 16585540; 16618810; 16767158; 17071630; 17085968; 17159907; 17450141; 17534194; 17623017; 17999750; 18381704; 18676680; 18716417; 18761323; 18823309; 19141860; 19166881; 19170196; 19219602; 19269008; 19367725; 19380486; 19414860; 19423537; 19427028; 19550122; 19604093; 19625176; 19692168; 19773279; 19784808; 19826114; 19861680; 19902255; 20402676; 20453000; 20467438; 20644561; 20661084; 20711500; 20800603; 20802294; 20829884; 20855536; 20959405; 20978178; 21368896; 21454616; 21559821; 21911414; 21988832; 22683265; 22782902; 22843554; 23212337; 23303631; 23541952; 23678861; 23921907; 25241761; 25330190; 25370148; 25403406; 25416956; 26164758; 26323380; 27799292; 28445729; 28643196; 29182622; 29322788; 31534141; 33049089; 34463927; |
| Motif | |
| Gene Encoded By | |
| Mass | 58,951 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.22.63; |