| IED ID | IndEnz0002004893 |
| Enzyme Type ID | protease004893 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase calypso EC 3.4.19.12 BAP1 homolog |
| Gene Name | calypso CG8445 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MNAAGGGSGAQAAAVAAGNNSLSHNALLSTASGATTMPMAQLADGWLELESDPGLFTLLLKDFGCHDVQVEEVYDLQKPIESPYGFIFLFRWIEERRARRKIVETTAEIFVKDEEAISSIFFAQQVVPNSCATHALLSVLLNCNENNLQLGDTLSRLKTHTKGMSPENKGLAIGNTPELACAHNSHAMPQARRRLERTGAGVSSCRFTGEAFHFVSFVPINGQLFELDGLKPYPMNHGGWEDSEDWTDKFRRVMAERLGIATGEQDIRFNLMAVVPDRRIAITHKLKMLRTNQAIVSGTLQKLLKADEQGESGNGDSQRPDTPTTLLEPSAFTARDLQSLLKNLDTEIAINEQHLADENDRRHMFKVDASRRTHNYDKFICTFLSMLAHQGVLGELVSQHLLPSKKVSGQGAANRISKQSTTASAGGSTAAGTASTPKTQQQQAAAAKNGKSPSKTPGRRRKGRNKCRKRK |
| Enzyme Length | 471 |
| Uniprot Accession Number | Q7K5N4 |
| Absorption | |
| Active Site | ACT_SITE 131; /note=Nucleophile; /evidence=ECO:0000305; ACT_SITE 213; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Polycomb group (PcG) protein. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-118' (H2AK118ub1). Does not deubiquitinate monoubiquitinated histone H2B. Required to maintain the transcriptionally repressive state of homeotic genes throughout development. The PR-DUB complex has weak or no activity toward 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. {ECO:0000269|PubMed:20436459}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (8); Chain (1); Compositional bias (3); Helix (12); Mutagenesis (1); Region (2); Site (1); Turn (2) |
| Keywords | 3D-structure;Chromatin regulator;Hydrolase;Nucleus;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | Q9V727 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus. Note=Localizes to PcG response elements (PREs). |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 6CGA; 6HGC; |
| Mapped Pubmed ID | 18460901; 19494831; 20005803; 20220848; 20371351; 21074052; 22096074; 22937016; 22994356; 23071443; 23275879; 23665228; 23899565; 24096405; 24671948; 25468754; 26120032; 26486609; 26876166; 26917723; 27172095; 27507044; 27557709; 28778878; 28927461; 29924323; 29995890; 30258054; 30349006; 30639226; 30873122; 31722958; 33105797; 33230107; 33456979; 33920158; 33988679; |
| Motif | |
| Gene Encoded By | |
| Mass | 51,507 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |