| IED ID | IndEnz0002004905 |
| Enzyme Type ID | protease004905 |
| Protein Name |
Cyanophycinase EC 3.4.15.6 Extracellular CGPase CPHEpa Extracellular cyanophycinase |
| Gene Name | cphE |
| Organism | Pseudomonas anguilliseptica |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa group Pseudomonas anguilliseptica |
| Enzyme Sequence | MIRSFIRSSALLLALLPVTGYSAGPLILVGGGLKDDNTAIYQRLIQLAGGNGQARIGVITAASIPESDDPDAGTADAANSKANGEFYAQLLETYGAADAQWIPIDLDQISNNSNPQVVAQINSMTGFFFGGGDQSRLTQTLQTATRADSPALAAIRARHNAGAVLAGTSAGTAIMVQGPMVTGGESYDGLRYGVYTTPSGDDLSYDMQGGFGFFNYGLLDTHFSERGRQGRIVRLADHTQVPFAFGVDENTALLVQNNATLGQVEMEVIGENGVFIFDLRNKERGTGSTYALYDVLGSYLTAGDRYRPVTGQFVIASGKTSLRGRERYSAAMTVTTDIFSSPNNSGANGRRKPREFVKVSADLFDSRVTSTLGRTYETNPLSRRSVQKHAVRQPWLPGHRWRQEHAVLPAFADGFPS |
| Enzyme Length | 417 |
| Uniprot Accession Number | Q8KQN8 |
| Absorption | |
| Active Site | ACT_SITE 169; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 188; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 222; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by serine protease inhibitors. Inhibited by N-Bromo-succinimide. {ECO:0000269|PubMed:11986309}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[L-4-(L-arginin-2-N-yl)aspartate](n) + H2O = [L-4-(L-arginin-2-N-yl)aspartate](n-1) + L-4-(L-arginin-2-N-yl)aspartate; Xref=Rhea:RHEA:12845, Rhea:RHEA-COMP:13728, Rhea:RHEA-COMP:13734, ChEBI:CHEBI:15377, ChEBI:CHEBI:137986, ChEBI:CHEBI:137991; EC=3.4.15.6; |
| DNA Binding | |
| EC Number | 3.4.15.6 |
| Enzyme Function | FUNCTION: Exopeptidase that catalyzes the hydrolytic cleavage of multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water-insoluble reserve polymer) into aspartate-arginine dipeptides. {ECO:0000269|PubMed:11986309, ECO:0000269|PubMed:14715021}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Signal peptide (1) |
| Keywords | Hydrolase;Protease;Secreted;Serine protease;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11986309}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 44,767 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.0 uM for cyanophycin granule polypeptide (CGP) {ECO:0000269|PubMed:14715021}; |
| Metal Binding | |
| Rhea ID | RHEA:12845 |
| Cross Reference Brenda | 3.4.15.6; |