| IED ID | IndEnz0002004917 |
| Enzyme Type ID | protease004917 |
| Protein Name |
Leishmanolysin EC 3.4.24.36 Cell surface protease Major surface glycoprotein Major surface protease Promastigote surface endopeptidase Protein gp63 |
| Gene Name | mspC |
| Organism | Leishmania tropica |
| Taxonomic Lineage | cellular organisms Eukaryota Discoba Euglenozoa Kinetoplastea (kinetoplasts) Metakinetoplastina Trypanosomatida Trypanosomatidae Leishmaniinae Leishmania Leishmania Leishmania tropica species complex Leishmania tropica |
| Enzyme Sequence | MSVDSSSSSTHRRRCVAARLVRLAAAGAAVTVAVGTAAAWAHAGALQHRCIHDAMQARVRQSVARHHTAPGAVSAVGLPYVTLDAAHTAAAADPRPGSAPTVVRAANWSTLRVAVSTEDLTDPAYHCARVGQRVNNHAGAIVTCTAEDILTDEKRDILRKYLIPQALQLHTERLKARQVQGKWKVTGMVDEICGDFKVPQAHITEGFSNTDFVMYVASVPSEEGVLAWATTCQVFSDGHPAVGVINIPAANIASRYDQLVTRVVTHEMAHALGFSEEFFTAARIVAHVSNVRHKTLKVPVVNSSTAVAKAREQYGCGTLEYLEIEDQGGAGSAGSHIKMRNAQDELMAPAAAGGYYTALTMAVFQDLGFYQADFNKAKVMPWGRNAGCAFLSEKCMEQNITKWRAMFCNESEDVMRCPTSRLSLGTCGIRGYRPPLPRYWQYFTNASLGGYSPFMDYCPVVIGYANGSCNQDASSAAEFLAAFNVFSEAARCIDGAFTPKNRTAADGYYAGLCANVRCDTATRTYSVQVRGSMDYVSCTPGLRVELSTVSNAFEEGGCITCPPYVEVCQGNVKGAKDFAGDSDSSSSADDAAGKAAMLRWNDRMVGLATAATVLLGMVLSLMALVVVWLLLVSCPWWCCKLGGPPASVTPACSPETE |
| Enzyme Length | 657 |
| Uniprot Accession Number | Q8MNZ1 |
| Absorption | |
| Active Site | ACT_SITE 267; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preference for hydrophobic residues at P1 and P1' and basic residues at P2' and P3'. A model nonapeptide is cleaved at -Ala-Tyr-|-Leu-Lys-Lys-.; EC=3.4.24.36; |
| DNA Binding | |
| EC Number | 3.4.24.36 |
| Enzyme Function | FUNCTION: Has an integral role during the infection of macrophages in the mammalian host. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (9); Glycosylation (7); Metal binding (3); Natural variant (1); Propeptide (1); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | Cell adhesion;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Signal;Transmembrane;Transmembrane helix;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..41; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 70,343 |
| Kinetics | |
| Metal Binding | METAL 266; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 270; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 336; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.36; |