| IED ID | IndEnz0002004938 |
| Enzyme Type ID | protease004938 |
| Protein Name |
Signal peptidase I SPase I EC 3.4.21.89 Leader peptidase I |
| Gene Name | lepB b2568 JW2552 |
| Organism | Escherichia coli (strain K12) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
| Enzyme Sequence | MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGDSLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFILVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDPVSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETKENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNRDNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGLRLSRIGGIH |
| Enzyme Length | 324 |
| Uniprot Accession Number | P00803 |
| Absorption | |
| Active Site | ACT_SITE 91; ACT_SITE 146 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.; EC=3.4.21.89; |
| DNA Binding | |
| EC Number | 3.4.21.89 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (19); Chain (1); Disulfide bond (1); Helix (6); Modified residue (1); Mutagenesis (20); Sequence conflict (6); Topological domain (3); Transmembrane (2); Turn (2) |
| Keywords | 3D-structure;Cell inner membrane;Cell membrane;Disulfide bond;Hydrolase;Membrane;Protease;Reference proteome;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:21778229}; Multi-pass membrane protein {ECO:0000269|PubMed:21778229}. |
| Modified Residue | MOD_RES 1; /note=Blocked amino end (Met); /evidence=ECO:0000269|PubMed:6311837 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (6) |
| Cross Reference PDB | 1B12; 1KN9; 1T7D; 3IIQ; 3S04; 6B88; |
| Mapped Pubmed ID | 11741964; 15136583; 19655811; 21999324; 30209367; |
| Motif | |
| Gene Encoded By | |
| Mass | 35,960 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.89; |