| IED ID | IndEnz0002004967 |
| Enzyme Type ID | protease004967 |
| Protein Name |
Mitochondrial-processing peptidase subunit beta EC 3.4.24.64 Beta-MPP Mitochondrial assembly protein 1 Processing enhancing protein PEP |
| Gene Name | MAS1 MIF1 YLR163C L9632.10 |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MFSRTASKFRNTRRLLSTISSQIPGTRTSKLPNGLTIATEYIPNTSSATVGIFVDAGSRAENVKNNGTAHFLEHLAFKGTQNRSQQGIELEIENIGSHLNAYTSRENTVYYAKSLQEDIPKAVDILSDILTKSVLDNSAIERERDVIIRESEEVDKMYDEVVFDHLHEITYKDQPLGRTILGPIKNIKSITRTDLKDYITKNYKGDRMVLAGAGAVDHEKLVQYAQKYFGHVPKSESPVPLGSPRGPLPVFCRGERFIKENTLPTTHIAIALEGVSWSAPDYFVALATQAIVGNWDRAIGTGTNSPSPLAVAASQNGSLANSYMSFSTSYADSGLWGMYIVTDSNEHNVQLIVNEILKEWKRIKSGKISDAEVNRAKAQLKAALLLSLDGSTAIVEDIGRQVVTTGKRLSPEEVFEQVDKITKDDIIMWANYRLQNKPVSMVALGNTSTVPNVSYIEEKLNQ |
| Enzyme Length | 462 |
| Uniprot Accession Number | P10507 |
| Absorption | |
| Active Site | ACT_SITE 73; /note="Proton acceptor"; /evidence="ECO:0000269|PubMed:11470436, ECO:0007744|PDB:1HR6" |
| Activity Regulation | ACTIVITY REGULATION: Binding to MAS2 is required for catalytic activity (PubMed:9654444, PubMed:9299349). Inhibited by high levels (> 1uM) of zinc (PubMed:9654444). Inhibited by metal chelators ethylenediaminetetraacetic acid (EDTA) and O-phenanthroline (PubMed:9654444). {ECO:0000269|PubMed:9299349, ECO:0000269|PubMed:9654444}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.; EC=3.4.24.64; Evidence={ECO:0000269|PubMed:2007593, ECO:0000269|PubMed:9299349, ECO:0000269|PubMed:9654444}; |
| DNA Binding | |
| EC Number | 3.4.24.64 |
| Enzyme Function | FUNCTION: Catalytic subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins (PubMed:2007593, PubMed:9299349, PubMed:9654444). Preferentially, cleaves after an arginine at position P2 (By similarity). {ECO:0000250|UniProtKB:Q03346, ECO:0000269|PubMed:2007593, ECO:0000269|PubMed:9299349, ECO:0000269|PubMed:9654444}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (15); Chain (1); Helix (21); Metal binding (3); Modified residue (1); Mutagenesis (10); Transit peptide (1); Turn (4) |
| Keywords | 3D-structure;Direct protein sequencing;Hydrolase;Metal-binding;Metalloprotease;Mitochondrion;Phosphoprotein;Protease;Reference proteome;Transit peptide;Zinc |
| Interact With | P11914 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000269|PubMed:3044780}. |
| Modified Residue | MOD_RES 243; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:18407956 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 1HR6; 1HR7; 1HR8; 1HR9; |
| Mapped Pubmed ID | 10449402; 10496979; 10545606; 10906275; 10942759; 11368022; 11805826; 11805837; 12191769; 12370023; 12791261; 1447206; 15458388; 16429126; 16554755; 17263664; 17445721; 19536198; 19930686; 20124346; 21525245; 22172993; 2229072; 22663076; 22984266; 24058582; 24336748; 24410772; 25176146; 25435547; 25973932; 26446170; 27398316; 2905264; 6235522; 7586024; 9050240; 9737975; 9829989; |
| Motif | |
| Gene Encoded By | |
| Mass | 51,084 |
| Kinetics | |
| Metal Binding | METAL 70; /note="Zinc; via tele nitrogen"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10096, ECO:0000269|PubMed:11470436, ECO:0000269|PubMed:9654444, ECO:0007744|PDB:1HR6, ECO:0007744|PDB:1HR7, ECO:0007744|PDB:1HR8, ECO:0007744|PDB:1HR9"; METAL 74; /note="Zinc; via tele nitrogen"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10096, ECO:0000269|PubMed:11470436, ECO:0007744|PDB:1HR6, ECO:0007744|PDB:1HR7, ECO:0007744|PDB:1HR8, ECO:0007744|PDB:1HR9"; METAL 150; /note="Zinc"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10096, ECO:0000269|PubMed:11470436, ECO:0007744|PDB:1HR6, ECO:0007744|PDB:1HR7, ECO:0007744|PDB:1HR8, ECO:0007744|PDB:1HR9" |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.64; |