| IED ID | IndEnz0002004995 |
| Enzyme Type ID | protease004995 |
| Protein Name |
Coagulation factor IX EC 3.4.21.22 Christmas factor Fragment |
| Gene Name | F9 |
| Organism | Cavia porcellus (Guinea pig) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Hystricomorpha Caviidae (cavies) Cavia (guinea pigs) Cavia porcellus (Guinea pig) |
| Enzyme Sequence | RVSIPSVSKEHNRANAIFSRMGYVNFTDDETIWDDNDDDETIWDNSTESTKPSDEFFRVVGGEDAKPGQFPWQVLLNGETEAFCGGSIVNEKWIVTAAHCILPGIKIEVVAGKHNIEKKEDTEQRRNVTQIILHHSYNASFNKYSHDIALLELDKPLSLNSYVTPICIANREYTNIFLKFGAGYVSGWGKLFSQGRTASILQYLRVPLVDRATCLRSTKFTIYNNMFCAGFHEGGRDSCQGDSGGPHVTEVEGTNFLTGIISWGEECAMKGKYGIYTKVSRYVNW |
| Enzyme Length | 285 |
| Uniprot Accession Number | P16295 |
| Absorption | |
| Active Site | ACT_SITE 99; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P00740; ACT_SITE 147; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P00740; ACT_SITE 243; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P00740 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.; EC=3.4.21.22; Evidence={ECO:0000250|UniProtKB:P00740}; |
| DNA Binding | |
| EC Number | 3.4.21.22 |
| Enzyme Function | FUNCTION: Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. {ECO:0000250|UniProtKB:P00740}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (3); Domain (1); Glycosylation (6); Metal binding (3); Modified residue (2); Non-terminal residue (2); Site (2) |
| Keywords | Blood coagulation;Calcium;Disulfide bond;Glycoprotein;Hemostasis;Hydrolase;Metal-binding;Phosphoprotein;Protease;Reference proteome;Secreted;Serine protease;Sulfation |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P00740}. |
| Modified Residue | MOD_RES 23; /note=Sulfotyrosine; /evidence=ECO:0000250|UniProtKB:P00740; MOD_RES 27; /note=Phosphothreonine; alternate; /evidence=ECO:0000250|UniProtKB:P00740 |
| Post Translational Modification | PTM: Activated by factor XIa, which excises the activation peptide. The propeptide can also be removed by snake venom protease. {ECO:0000250|UniProtKB:P00740}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 32,032 |
| Kinetics | |
| Metal Binding | METAL 115; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P00740; METAL 120; /note=Calcium; /evidence=ECO:0000250|UniProtKB:P00740; METAL 123; /note=Calcium; /evidence=ECO:0000250|UniProtKB:P00740 |
| Rhea ID | |
| Cross Reference Brenda |