| IED ID | IndEnz0002005001 |
| Enzyme Type ID | protease005001 |
| Protein Name |
Cystatin-like cysteine protease inhibitor EPIC2B Extracellular protease inhibitor with cystatin-like domain protein 2B Secreted effector EPIC2B |
| Gene Name | EPIC2B PITG_09173 |
| Organism | Phytophthora infestans (strain T30-4) (Potato late blight fungus) |
| Taxonomic Lineage | cellular organisms Eukaryota Sar Stramenopiles Oomycota Peronosporales Peronosporaceae Phytophthora Phytophthora infestans (Potato late blight agent) (Botrytis infestans) Phytophthora infestans (strain T30-4) (Potato late blight fungus) |
| Enzyme Sequence | MSFLRPTLALLAVTALVTTSAQLNGYSKKEVTPEDTELLQKAQSNVSAYNSDVTSRICYLKVDSLETQVVSGENYKFHVSGCSVNSDKELGGCANQNCESSKYDIVIYSQSWTNTLKVTSITPAN |
| Enzyme Length | 125 |
| Uniprot Accession Number | D0NBV3 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Secreted effector that interacts with and inhibits the pathogenesis-related papain-like cysteine proteases C14, PIP1 and RCR3 of host plants (PubMed:17085509, PubMed:19171904, PubMed:20940351). Inhibition of host proteases by a pathogen extracellular protease inhibitor forms a specific type of defense-counterdefense mechanism between plants and microbial pathogens (PubMed:17085509, PubMed:19171904, PubMed:20940351). {ECO:0000269|PubMed:17085509, ECO:0000269|PubMed:19171904, ECO:0000269|PubMed:20940351}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Glycosylation (1); Motif (1); Signal peptide (1); Site (1) |
| Keywords | Glycoprotein;Protease inhibitor;Reference proteome;Secreted;Signal;Thiol protease inhibitor;Virulence |
| Interact With | |
| Induction | INDUCTION: Expression is up-regulated during infection of host tomato. {ECO:0000269|PubMed:17085509}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17085509, ECO:0000269|PubMed:19171904, ECO:0000269|PubMed:20940351}. Note=Localizes to host apoplast where it targets defense proteases for inhibition. {ECO:0000269|PubMed:17085509, ECO:0000269|PubMed:19171904, ECO:0000269|PubMed:20940351}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 68..72; /note=Secondary area of contact; /evidence=ECO:0000250|UniProtKB:P01040 |
| Gene Encoded By | |
| Mass | 13,610 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |