| IED ID | IndEnz0002005061 |
| Enzyme Type ID | protease005061 |
| Protein Name |
Probable cytosol aminopeptidase EC 3.4.11.1 Leucine aminopeptidase LAP EC 3.4.11.10 Leucyl aminopeptidase |
| Gene Name | pepA pepB Cgl2205 cg2419 |
| Organism | Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Corynebacteriaceae Corynebacterium Corynebacterium glutamicum (Brevibacterium saccharolyticum) Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) |
| Enzyme Sequence | MSKDATLPVRGTVAELKLEKKLPKKIDAIIVAIFEGEDSIELAGGEILDFIFSTEQQADILTQLEAVGAKATANSITRVPGTDVAPVIAVGLGKADLLDDETLRRASGTAARSLGGFENVATTIGDLGLAAAVTGFGLGSYSYAGLRKETEESKDKTTTVTFISTGKDDKDVFVEAQIIVESVLLARDLVNTPSSHLYPESYSVIASNEASKHGLQTTILDEKQLADQGFGGILAVGNGSSRKPRLLRIDWKPRKAKKSIALVGKGITFDTGGISIKPGASMENMISDMGGSASVLATIIAAARLNLSINVSAFLPMAENMPSGDAFRPGDVITHFGGITSEILNTDAEGRLILADAIAYASEDKPDYLIDAATLTGAQLVALGLRTSGVMGTDEFRDSVAKTGREVGEQAWAMPLPEELDEQVKSPVADLRNVTNSRFAGMSAAGRYLQEFVGADIEWAHVDIAGPAYNTAGEFGYTPKRATGQPVRTFVQVLKDLSES |
| Enzyme Length | 500 |
| Uniprot Accession Number | Q8NNJ4 |
| Absorption | |
| Active Site | ACT_SITE 277; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 351; /evidence=ECO:0000255|HAMAP-Rule:MF_00181 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; |
| DNA Binding | |
| EC Number | 3.4.11.1; 3.4.11.10 |
| Enzyme Function | FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Erroneous initiation (1); Metal binding (7) |
| Keywords | Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 52,788 |
| Kinetics | |
| Metal Binding | METAL 265; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 270; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 270; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 288; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 347; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 349; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 349; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181 |
| Rhea ID | |
| Cross Reference Brenda |