| IED ID | IndEnz0002005075 |
| Enzyme Type ID | protease005075 |
| Protein Name |
Cathepsin L EC 3.4.22.15 Cysteine proteinase 1 Cleaved into: Cathepsin L heavy chain; Cathepsin L light chain |
| Gene Name | Cp1 fs(2)50Ca CG6692 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MNHLGVFETRFRPRTRHKSQRAQLIPEQITMRTAVLLPLLALLAVAQAVSFADVVMEEWHTFKLEHRKNYQDETEERFRLKIFNENKHKIAKHNQRFAEGKVSFKLAVNKYADLLHHEFRQLMNGFNYTLHKQLRAADESFKGVTFISPAHVTLPKSVDWRTKGAVTAVKDQGHCGSCWAFSSTGALEGQHFRKSGVLVSLSEQNLVDCSTKYGNNGCNGGLMDNAFRYIKDNGGIDTEKSYPYEAIDDSCHFNKGTVGATDRGFTDIPQGDEKKMAEAVATVGPVSVAIDASHESFQFYSEGVYNEPQCDAQNLDHGVLVVGFGTDESGEDYWLVKNSWGTTWGDKGFIKMLRNKENQCGIASASSYPLV |
| Enzyme Length | 371 |
| Uniprot Accession Number | Q95029 |
| Absorption | |
| Active Site | ACT_SITE 178; /evidence=ECO:0000250; ACT_SITE 317; /evidence=ECO:0000250; ACT_SITE 338; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.; EC=3.4.22.15; |
| DNA Binding | |
| EC Number | 3.4.22.15 |
| Enzyme Function | FUNCTION: Important for the overall degradation of proteins in lysosomes. Essential for adult male and female fertility. May play a role in digestion. {ECO:0000269|PubMed:7851441, ECO:0000269|PubMed:9099581, ECO:0000269|PubMed:9662479}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (1); Chain (2); Disulfide bond (3); Glycosylation (1); Propeptide (2); Sequence caution (1); Sequence conflict (4); Signal peptide (1) |
| Keywords | Alternative splicing;Developmental protein;Digestion;Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Protease;Reference proteome;Signal;Thiol protease;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Lysosome {ECO:0000269|PubMed:9099581}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..48; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10471706; 10511707; 10580984; 10704400; 12408803; 12408844; 12417656; 12593804; 12743107; 12765609; 12885559; 13130081; 14605208; 14630943; 14647296; 14668392; 14737191; 15573123; 15731005; 16678775; 16797177; 17194782; 17409713; 17998402; 18067683; 18355804; 19057646; 19064709; 19079254; 19214204; 19321433; 19951294; 20220848; 20371351; 20813047; 20832315; 21074052; 21535339; 21923734; 22150223; 22174071; 22426392; 22450469; 22884528; 22934826; 23028818; 23071443; 23256878; 23408904; 23583758; 23810935; 23968895; 24114784; 24302888; 24553932; 24554766; 24582961; 24703951; 25000897; 25173815; 25312911; 25422373; 25776889; 25848931; 25970330; 26092939; 26220534; 26224590; 26341534; 26405051; 26431669; 26526100; 26550828; 26839216; 26870755; 26998322; 27141974; 27582081; 27648494; 27794539; 27852225; 27924562; 28067623; 28369070; 28484036; 28693471; 29066546; 29452635; 29551411; 29940804; 30012668; 30111579; 30741620; 30943394; 31001531; 31285547; 31286069; 31674908; 31722958; 32286230; 32339165; 32915229; 32993812; 33112206; 33300868; 33543035; 33748138; 33863891; 34200604; 8978048; 8978049; 8978050; 9619103; |
| Motif | |
| Gene Encoded By | |
| Mass | 41,601 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |