| IED ID | IndEnz0002005103 |
| Enzyme Type ID | protease005103 |
| Protein Name |
Calpain-9 EC 3.4.22.- Digestive tract-specific calpain New calpain 4 nCL-4 |
| Gene Name | Capn9 Ncl4 |
| Organism | Rattus norvegicus (Rat) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat) |
| Enzyme Sequence | MPYLHRSLRPQPQPVPRDARTVHSSGHSFEQLRQSCLQSGTLFEDADFPASNSSLFYSERPQVPFVWKRPGEIVENPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALARVVPQDQGFGSGYAGIFHFQFWQHSEWLDVVIDDRLPTFRDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAENFQIREAPEDFYEILEKALRRGSLLGCSIDTLNASESEARTPLGLIKGHAYTVTGLDQVNFHGQRIKLIRVRNPWGQVEWNGPWSDSSPEWRSMSLEEQKRLGHTALDDGEFWMAFEDFKTHFDKVEICNLTPDALEDNTLHKWEVTIHQGSWVRGSTAGGCRNFLDTFWTNPQIKLSLTERDEGQEGCTFLAALMQKDRRRLKRFGANMLTIGYAIYQCPDKDGHLNRDFFRYHASLARSKTFINLREVSGRFQLPPGDYILIPSTFEPHQEADFCLRIFSEKKAVTQDLDENIDIDLPELPKPTPQEEETEEERQFRALFRRIAGEDMEVSAEELEYVLNAVLQKKTALKFKRLSLLSCRNIISLMDTSGNGKMEFEEFRVFWDKLRYWMDLFLQFDVDKSGTMSSYELRTALKAAGFQLGSHLLQLIVLRYADENLQLDFDDYLNCLVRLENASRVFQCLSVKNKDFIHLNINEFINLTMNI |
| Enzyme Length | 690 |
| Uniprot Accession Number | O35920 |
| Absorption | |
| Active Site | ACT_SITE 97; /evidence=ECO:0000250; ACT_SITE 254; /evidence=ECO:0000250; ACT_SITE 278; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Calcium-regulated non-lysosomal thiol-protease. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (4); Metal binding (19); Region (3); Sequence conflict (2) |
| Keywords | Calcium;Hydrolase;Metal-binding;Protease;Reference proteome;Repeat;Thiol protease |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15665521; |
| Motif | |
| Gene Encoded By | |
| Mass | 79,263 |
| Kinetics | |
| Metal Binding | METAL 81; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250; METAL 83; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250; METAL 88; /note=Calcium 1; /evidence=ECO:0000250; METAL 167; /note=Calcium 1; /evidence=ECO:0000250; METAL 284; /note=Calcium 2; /evidence=ECO:0000250; METAL 291; /note=Calcium 2; /evidence=ECO:0000250; METAL 312; /note=Calcium 2; via carbonyl oxygen; METAL 314; /note=Calcium 2; /evidence=ECO:0000250; METAL 316; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000250; METAL 574; /note=Calcium 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 576; /note=Calcium 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 578; /note=Calcium 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 580; /note=Calcium 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 585; /note=Calcium 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 604; /note=Calcium 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 606; /note=Calcium 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 608; /note=Calcium 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 610; /note=Calcium 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; METAL 615; /note=Calcium 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.22.B29; |