| IED ID | IndEnz0002005153 |
| Enzyme Type ID | protease005153 |
| Protein Name |
Major capsid protein Capsid protein pb8 Major head protein Cleaved into: Scaffolding domain delta |
| Gene Name | D20 ORF138 T5.149 T5p145 |
| Organism | Escherichia phage T5 (Enterobacteria phage T5) |
| Taxonomic Lineage | Viruses Duplodnaviria Heunggongvirae Uroviricota Caudoviricetes Caudovirales Demerecviridae Markadamsvirinae Tequintavirus Escherichia phage T5 (Enterobacteria phage T5) |
| Enzyme Sequence | MTIDINKLKEELGLGDLAKSLEGLTAAQKAQEAERMRKEQEEKELARMNDLVSKAVGEDRKRLEEALELVKSLDEKSKKSNELFAQTVEKQQETIVGLQDEIKSLLTAREGRSFVGDSVAKALYGTQENFEDEVEKLVLLSYVMEKGVFETEHGQRHLKAVNQSSSVEVSSESYETIFSQRIIRDLQKELVVGALFEELPMSSKILTMLVEPDAGKATWVAASTYGTDTTTGEEVKGALKEIHFSTYKLAAKSFITDETEEDAIFSLLPLLRKRLIEAHAVSIEEAFMTGDGSGKPKGLLTLASEDSAKVVTEAKADGSVLVTAKTISKLRRKLGRHGLKLSKLVLIVSMDAYYDLLEDEEWQDVAQVGNDSVKLQGQVGRIYGLPVVVSEYFPAKANSAEFAVIVYKDNFVMPRQRAVTVERERQAGKQRDAYYVTQRVNLQRYFANGVVSGTYAAS |
| Enzyme Length | 458 |
| Uniprot Accession Number | Q6QGD8 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Major capsid protein that self-associates to form 120 hexamers and 11 pentamers, building the T=13 icosahedral capsid which about 860 Angstroms in diameter. Responsible for its self-assembly into a procapsid. The phage does not need to encode a separate scaffolfing protein because its capsid protein contains the delta domain that carries that function. The capsid gains its final stability through the reorganization of the subunits that takes place upon expansion. DNA encapsidation through the portal triggers capsid expansion and the binding of the decoration protein to the capsid exterior. Might play a role in counteracting the host Pycsar defense system that is mediated by pyrimidine cyclases and leads to abortive infection. {ECO:0000269|PubMed:20573812, ECO:0000269|PubMed:23500494, ECO:0000269|PubMed:26616586, ECO:0000269|PubMed:34644530}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (2); Coiled coil (1); Erroneous initiation (1); Mutagenesis (8); Site (1) |
| Keywords | 3D-structure;Capsid protein;Coiled coil;Late protein;Reference proteome;T=13 icosahedral capsid protein;Viral capsid assembly;Viral release from host cell;Virion |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:24198424, ECO:0000269|PubMed:4571380}. Note=Forms the icosahedral capsid shell which contains 775 major capsid proteins. {ECO:0000269|PubMed:4571380}. |
| Modified Residue | |
| Post Translational Modification | PTM: The scaffolding domain delta is cleaved by the prohead protease and lost after assembly (PubMed:16876823). The major capsid protein precursors together with both the portal complex and the maturation protease form prohead I (PubMed:26616586). All copies of the major capsid protein precursor are cleaved to the mature major capsid protein by release of the scaffolding domain delta, yielding the metastable prohead II (PubMed:26616586). {ECO:0000269|PubMed:16876823, ECO:0000269|PubMed:26616586}. |
| Signal Peptide | |
| Structure 3D | Electron microscopy (4) |
| Cross Reference PDB | 5TJT; 6OKB; 6OMA; 6OMC; |
| Mapped Pubmed ID | 28165000; 31578255; |
| Motif | |
| Gene Encoded By | |
| Mass | 50,885 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |