| IED ID | IndEnz0002005190 |
| Enzyme Type ID | protease005190 |
| Protein Name |
Calpain-3 EC 3.4.22.54 Calcium-activated neutral proteinase 3 CANP 3 Calpain L3 Calpain p94 Muscle-specific calcium-activated neutral protease 3 |
| Gene Name | CAPN3 |
| Organism | Gallus gallus (Chicken) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Archelosauria Archosauria Dinosauria Saurischia Theropoda Coelurosauria Aves Neognathae Galloanserae Galliformes Phasianidae (turkeys) Phasianinae Gallus Gallus gallus (Chicken) |
| Enzyme Sequence | MPSAINAAVAQQTAAGSVPSTTSTTTEGTGGGTGGIYSAIISRNQPIIKVKEKTYEELHKKCLEENILYEDPDFPPNETSLFYSQKVPIKFEWKRPREICENPRFIIGGANRTDICQGELGDCWFLAAIACLTLNKKLLCRVIPHDQSFIQNYAGIFHFQFWRYGDWVDVIIDDCLPTYNNQLVFTKSSQRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVIEFYEIKDAPKDIYKIMKHAIARGSLMASSIDDNLGFHYGAAPRSDIGELIARMVKNLENAQMTYSTVDYQGTDERPAWTIMPMQYETRMSCGLVKGHAYSVTAVEETTYKGEKMRLVRLRNPWGQVEWNGPWSDKSEEWNFIDEEEKIRLQHKIAEDGEFWISLEDFMRHFTKLEICNLTPDTLEADKLQTWTVSVNEGRWVRGCSAGGCRNYPDTFWTNPQYRLKLLEEDDDPEDEEVICSFLVALMQKNRRKERKLGANLYTIGFAIYEVPKEMHGTKHHLQKDFFLYNASKARSKTYINMREISERFRLPPSEYVIIPSTYEPHQEGEFILRVFSEKRSLSEEVENMIEADRPSKKKKGKPIIFVSDRANSNKELTTDEDAGKDGEKTHVDEKKRSSAKAREKSEEETQFRNIFRQIAGDDMEICREELRNVLNNVVKKHKDLKTEGFELESSRSMIALMDTDGSGKINFDEFRHLWDKIKSWQKIFKHYDADHSGTINSYEMRNAVKDAGFRLNNQLYDIITMRYADKNMNIDFDSFICCFVRLDAMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA |
| Enzyme Length | 810 |
| Uniprot Accession Number | Q92177 |
| Absorption | |
| Active Site | ACT_SITE 123; /evidence=ECO:0000255|PROSITE-ProRule:PRU00239; ACT_SITE 327; /evidence=ECO:0000255|PROSITE-ProRule:PRU00239; ACT_SITE 351; /evidence=ECO:0000255|PROSITE-ProRule:PRU00239 |
| Activity Regulation | ACTIVITY REGULATION: Activated by micromolar concentrations of calcium and inhibited by calpastatin. |
| Binding Site | |
| Calcium Binding | CA_BIND 651..661; /note="1"; /evidence="ECO:0000250|UniProtKB:P20807"; CA_BIND 694..705; /note="2"; /evidence="ECO:0000250|UniProtKB:P20807, ECO:0000255|PROSITE-ProRule:PRU00448"; CA_BIND 724..730; /note="3"; /evidence="ECO:0000250|UniProtKB:P20807, ECO:0000255|PROSITE-ProRule:PRU00448"; CA_BIND 789..795; /note="4"; /evidence="ECO:0000250|UniProtKB:P20807" |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Broad endopeptidase activity.; EC=3.4.22.54; |
| DNA Binding | |
| EC Number | 3.4.22.54 |
| Enzyme Function | FUNCTION: Calcium-regulated non-lysosomal thiol-protease. Proteolytically cleaves CTBP1. Mediates, with UTP25, the proteasome-independent degradation of p53/TP53. {ECO:0000250|UniProtKB:P20807}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Calcium binding (4); Chain (1); Compositional bias (2); Domain (5); Region (5) |
| Keywords | Calcium;Cytoplasm;Hydrolase;Metal-binding;Nucleus;Protease;Reference proteome;Repeat;Thiol protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P20807}. Nucleus, nucleolus {ECO:0000250|UniProtKB:P20807}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 19265533; 21713405; 32932207; |
| Motif | |
| Gene Encoded By | |
| Mass | 93,561 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.22.54; |