Detail Information for IndEnz0002005377
IED ID IndEnz0002005377
Enzyme Type ID protease005377
Protein Name Vignain
EC 3.4.22.-
Bean endopeptidase
Cysteine proteinase EP-C1
Gene Name
Organism Phaseolus vulgaris (Kidney bean) (French bean)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Fabales Fabaceae Papilionoideae 50 kb inversion clade NPAAA clade indigoferoid/millettioid clade Phaseoleae Phaseolus Phaseolus vulgaris (Kidney bean) (French bean)
Enzyme Sequence MATKKLLWVVLSFSLVLGVANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEKHKRFNVFKANLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRGTPHENGAFMYEKVVSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQELVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGHENVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCSTDLNHGVAIVGYGTTVDGTNYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPIKNSSDNPTGSFSSPKDEL
Enzyme Length 362
Uniprot Accession Number P25803
Absorption
Active Site ACT_SITE 152; /evidence=ECO:0000250; ACT_SITE 288; /evidence=ECO:0000250; ACT_SITE 309; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.22.-
Enzyme Function FUNCTION: Thought to be involved in the hydrolysis of stored seed proteins.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Glycosylation (2); Motif (1); Propeptide (1); Sequence conflict (1); Signal peptide (1)
Keywords Endoplasmic reticulum;Glycoprotein;Hydrolase;Protease;Signal;Thiol protease;Zymogen
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Endoplasmic reticulum lumen.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..20; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif MOTIF 359..362; /note=Prevents secretion from ER; /evidence=ECO:0000255|PROSITE-ProRule:PRU10138
Gene Encoded By
Mass 40,212
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda