Detail Information for IndEnz0002005384
IED ID IndEnz0002005384
Enzyme Type ID protease005384
Protein Name COP9 signalosome complex subunit 5b
Signalosome subunit 5b
EC 3.4.-.-
Jun activation domain-binding homolog 2
Gene Name CSN5B AJH2 CSN5A At1g71230 F3I17.12
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MEGSSSTIARKTWELENSILTVDSPDSTSDNIFYYDDTSQTRFQQEKPWENDPHYFKRVKISALALLKMVVHARSGGTIEIMGLMQGKTDGDTIIVMDAFALPVEGTETRVNAQDDAYEYMVEYSQTNKLAGRLENVVGWYHSHPGYGCWLSGIDVSTQRLNQQHQEPFLAVVIDPTRTVSAGKVEIGAFRTYSKGYKPPDEPVSEYQTIPLNKIEDFGVHCKQYYSLDVTYFKSSLDSHLLDLLWNKYWVNTLSSSPLLGNGDYVAGQISDLAEKLEQAESHLVQSRFGGVVPSSLHKKKEDESQLTKITRDSAKITVEQVHGLMSQVIKDELFNSMRQSNNKSPTDSSDPDPMITY
Enzyme Length 358
Uniprot Accession Number Q9FVU9
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.-.-
Enzyme Function FUNCTION: Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes such as photomorphogenesis and auxin and jasmonate responses. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex (By similarity). The CSN complex is involved in repression of photomorphogenesis in darkness by regulating the activity of COP1-containing Ubl ligase complexes. The complex is also required for degradation of PSIAA6 by regulating the activity of the Ubl ligase SCF-TIR complex. Not involved in CSN's deneddylation/derubylation activity (PubMed:15486099, PubMed:17307927). Essential for the structural integrity of the CSN holocomplex (PubMed:17307927). {ECO:0000250, ECO:0000269|PubMed:11337587, ECO:0000269|PubMed:15486099, ECO:0000269|PubMed:17307927, ECO:0000269|PubMed:9811788}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Domain (1); Metal binding (3); Modified residue (1); Motif (1); Mutagenesis (5); Region (1); Sequence conflict (1)
Keywords Acetylation;Cytoplasm;Developmental protein;Hydrolase;Metal-binding;Metalloprotease;Nucleus;Phytochrome signaling pathway;Protease;Reference proteome;Signalosome;Zinc
Interact With Q9ZT84; Q9LYC1; O82255; P93830; Q9SZ67
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:9811788}. Nucleus {ECO:0000269|PubMed:9811788}.
Modified Residue MOD_RES 1; /note=N-acetylmethionine; /evidence=ECO:0000250|UniProtKB:Q8LAZ7
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 11019806; 11701877; 12223399; 12724534; 14570571; 15923347; 16844902; 18434413; 18467458; 19305390; 19847120; 20036030; 20083325; 21441437; 21798944; 23086934; 23603957; 24286178; 24908506; 27561782; 29193661; 9707402;
Motif MOTIF 142..155; /note=JAMM motif; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182
Gene Encoded By
Mass 40,318
Kinetics
Metal Binding METAL 142; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182; METAL 144; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182; METAL 155; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182
Rhea ID
Cross Reference Brenda