| IED ID |
IndEnz0002005390 |
| Enzyme Type ID |
protease005390 |
| Protein Name |
Cyclotide trypsin inhibitor TopI1
|
| Gene Name |
|
| Organism |
Tityus obscurus (Amazonian scorpion) (Tityus cambridgei) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Metazoa
Eumetazoa
Bilateria
Protostomia
Ecdysozoa
Panarthropoda
Arthropoda
Chelicerata
Arachnida
Scorpiones
Buthida
Buthoidea
Buthidae
Tityus
Tityus obscurus (Amazonian scorpion) (Tityus cambridgei)
|
| Enzyme Sequence |
MKFIIVLLLLTALTLTSIPVIEGILKRCKTYDDCKDVCKARKGKCEFGICKCMIKSGK |
| Enzyme Length |
58 |
| Uniprot Accession Number |
A0A6P3CW73 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
|
| EC Number |
|
| Enzyme Function |
FUNCTION: First cyclic scorpion trypsin inhibitor (Kd~0.5 nM). Does not inhibit chymotrypsin. {ECO:0000269|PubMed:32787139}. |
| Temperature Dependency |
BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Is very stable at high temperature. {ECO:0000269|PubMed:32787139}; |
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Chain (1); Cross-link (1); Disulfide bond (3); Modified residue (1); Mutagenesis (1); Signal peptide (1); Site (2) |
| Keywords |
3D-structure;Amidation;Direct protein sequencing;Disulfide bond;Knottin;Protease inhibitor;Secreted;Serine protease inhibitor;Signal |
| Interact With |
|
| Induction |
|
| Subcellular Location |
SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:32787139}. |
| Modified Residue |
MOD_RES 56; /note=Serine amide; /evidence=ECO:0000269|PubMed:32787139 |
| Post Translational Modification |
PTM: This is a cyclic peptide. {ECO:0000269|PubMed:32787139}. |
| Signal Peptide |
SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D |
X-ray crystallography (1) |
| Cross Reference PDB |
6MRQ;
|
| Mapped Pubmed ID |
- |
| Motif |
|
| Gene Encoded By |
|
| Mass |
6,482 |
| Kinetics |
|
| Metal Binding |
|
| Rhea ID |
|
| Cross Reference Brenda |
|