| IED ID | IndEnz0002005430 |
| Enzyme Type ID | protease005430 |
| Protein Name |
mRNA-decapping enzyme 1A EC 3.6.1.62 Smad4-interacting transcriptional co-activator Transcription factor SMIF |
| Gene Name | DCP1A SMIF |
| Organism | Sus scrofa (Pig) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Suina Suidae (pigs) Sus Sus scrofa (Pig) |
| Enzyme Sequence | MESLSRAGQEMSLAALKQHDPYITSIADLTGQVALYTFCPKANQWEKTDIEGTLFVYRRSASPYHGFTIVNRLNMHNLVEPVNKDLEFQLHEPFLLYRNASLSIYSIWFYDKNDCHRIAKLMADVLEEETRRSQQAARDKQSPNQANGCSDHRPIDILEMLSRAKDEYERNQMGDSNISSPGLQPSTQISNLGSTETLEETPSGLQDKSALSGHKHLTVEELFGTSLPKEQPTVVGLESEEVEKLPGDASQKEPSSFLPFSFEPSGGGPQSENMGIRPAAHHSVQPEVTTPVLITPASITQSSEKQAPSYAIPLHPVLSPTLPAEASTAQAPPSLPRSTTMMQAVKTTPRQRSPLSSQPVPELSQASLAASQSPFRAPLNVTNTASTSLPSVDLLQKLRLTQQHDQIQTQSLGKGAVAPSFSPAAGQLATPESFIEPPPKTAAARASASLSNMVLAPLQSMQQNQDPEVFAQPKVLSSAIPVAGPALVTATTSAVSSVLLSPSVFQQTVTRSSDLERKASSPSPLTVGTSENQRKPSIILSKSQLQDTLIHLIKNDSSFLSTLHEVYLQVLTKNKDNHNL |
| Enzyme Length | 580 |
| Uniprot Accession Number | I3LHS8 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + 2 H(+) + N(7)-methyl-GDP; Xref=Rhea:RHEA:67484, Rhea:RHEA-COMP:15692, Rhea:RHEA-COMP:17167, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:63714, ChEBI:CHEBI:138282, ChEBI:CHEBI:156461; EC=3.6.1.62; Evidence={ECO:0000250|UniProtKB:Q9NPI6};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67485; Evidence={ECO:0000250|UniProtKB:Q9NPI6}; |
| DNA Binding | |
| EC Number | 3.6.1.62 |
| Enzyme Function | FUNCTION: Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1 (By similarity). Essential for embryonic development (By similarity). {ECO:0000250|UniProtKB:Q9NPI6}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (1); Modified residue (16); Region (5); Site (1) |
| Keywords | Cytoplasm;Hydrolase;Methylation;Nonsense-mediated mRNA decay;Nucleus;Phosphoprotein;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm, P-body {ECO:0000250|UniProtKB:Q9NPI6}. Nucleus {ECO:0000250|UniProtKB:Q9NPI6}. Note=Co-localizes with NANOS3 in the processing bodies (By similarity). Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4 (By similarity). {ECO:0000250|UniProtKB:Q91YD3, ECO:0000250|UniProtKB:Q9NPI6}. |
| Modified Residue | MOD_RES 62; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q9NPI6; MOD_RES 142; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q9NPI6; MOD_RES 179; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q91YD3; MOD_RES 180; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q9NPI6; MOD_RES 319; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q9NPI6; MOD_RES 334; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q9NPI6; MOD_RES 348; /note=Phosphothreonine; /evidence=ECO:0000250|UniProtKB:Q9NPI6; MOD_RES 353; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q9NPI6; MOD_RES 376; /note=Asymmetric dimethylarginine; /evidence=ECO:0000250|UniProtKB:Q9NPI6; MOD_RES 401; /note=Phosphothreonine; /evidence=ECO:0000250|UniProtKB:Q9NPI6; MOD_RES 422; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q9NPI6; MOD_RES 520; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q9NPI6; MOD_RES 521; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q9NPI6; MOD_RES 523; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:Q9NPI6; MOD_RES 526; /note=Phosphothreonine; /evidence=ECO:0000250|UniProtKB:Q91YD3; MOD_RES 529; /note=Phosphothreonine; /evidence=ECO:0000250|UniProtKB:Q9NPI6 |
| Post Translational Modification | PTM: (Microbial infection) Cleaved by porcine reproductive and respiratory syndrome virus serine protease nsp4 after Glu-238. The cleavage inhibits DCP1A function. {ECO:0000269|PubMed:30158128}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 62,945 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:67484; RHEA:67485 |
| Cross Reference Brenda |