| IED ID | IndEnz0002005460 |
| Enzyme Type ID | protease005460 |
| Protein Name |
Blarina toxin BLTX EC 3.4.21.- |
| Gene Name | BTX |
| Organism | Blarina brevicauda (Northern short-tailed shrew) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Eulipotyphla (hedgehogs shrews moles and others) Soricidae (shrews) Soricinae (red-toothed shrews) Blarina Blarina brevicauda (Northern short-tailed shrew) |
| Enzyme Sequence | MCFLLLCLTLTLAGTGAVPTGPSIEIHSRIIGGWECDKHSQPWQALLTFTRKHNSVCGGVLVHSQWVLTAAHCIGDNYKVLLGLHDRSSEESTVQEARVSARFPHPLYNMTLLNLLLSHKMNLTFFYKTFLGADFSHDLMLLRLDQPVQLTDAVQVLDLPTQEPQVGSTCHVSGWGRTSQNYENSFVLPEKLQCVEFTLLSNNECSHAHMFKVTEAMLCAGHMEGGKDSCVGDSGGPLICDGVFQGIASWGSSPCGQQGRPGIYVKVFLYISWIQETIKAHS |
| Enzyme Length | 282 |
| Uniprot Accession Number | Q76B45 |
| Absorption | |
| Active Site | ACT_SITE 72; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 138; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 234; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | ACTIVITY REGULATION: Strongly inhibited by aprotinin, moderately inhibited by secretory leukoprotease inhibitor, the Kunitz-type soybean trypsin inhibitor, and leupeptin, and not inhibited by urinary trypsin inhibitor or alpha-1 protease inhibitor. {ECO:0000269|PubMed:15136743}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Has kallikrein-like activity, converts kininogens to kinins, and has dilatory effects on the blood vessel walls. Shows highest activity toward Pro-Phe-Arg-MCA and Boc-Val-Leu-Lys-MCA in vitro. Has preference for Arg and Lys in position P1 and hydrophobic residues in position P2. {ECO:0000269|PubMed:15136743}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 9.0. {ECO:0000269|PubMed:15136743}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (5); Domain (1); Glycosylation (3); Propeptide (1); Signal peptide (1) |
| Keywords | Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Protease;Secreted;Serine protease;Signal;Toxin;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 31,066 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |