| IED ID | IndEnz0002005557 |
| Enzyme Type ID | protease005557 |
| Protein Name |
Rhomboid protease GlpG EC 3.4.21.105 Intramembrane serine protease |
| Gene Name | glpG b3424 JW5687 |
| Organism | Escherichia coli (strain K12) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
| Enzyme Sequence | MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQAERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMIACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWYLGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERDPQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK |
| Enzyme Length | 276 |
| Uniprot Accession Number | P09391 |
| Absorption | |
| Active Site | ACT_SITE 201; /note="Nucleophile"; /evidence="ECO:0000305|PubMed:17051161, ECO:0000305|PubMed:17099694, ECO:0000305|PubMed:17190827, ECO:0000305|PubMed:17277078"; ACT_SITE 254; /evidence="ECO:0000305|PubMed:17051161, ECO:0000305|PubMed:17099694, ECO:0000305|PubMed:17190827, ECO:0000305|PubMed:17277078" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.; EC=3.4.21.105; |
| DNA Binding | |
| EC Number | 3.4.21.105 |
| Enzyme Function | FUNCTION: Rhomboid-type serine protease that catalyzes intramembrane proteolysis. {ECO:0000269|PubMed:16216077, ECO:0000269|PubMed:17099694}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (4); Chain (1); Erroneous initiation (1); Helix (15); Mutagenesis (4); Sequence conflict (8); Topological domain (7); Transmembrane (6); Turn (2) |
| Keywords | 3D-structure;Cell inner membrane;Cell membrane;Direct protein sequencing;Hydrolase;Membrane;Protease;Reference proteome;Serine protease;Transmembrane;Transmembrane helix |
| Interact With | Itself |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:16216077}; Multi-pass membrane protein {ECO:0000269|PubMed:16216077}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | NMR spectroscopy (1); X-ray crystallography (43) |
| Cross Reference PDB | 2IC8; 2IRV; 2LEP; 2NRF; 2O7L; 2XOV; 2XOW; 2XTU; 2XTV; 3B44; 3B45; 3TXT; 3UBB; 3ZEB; 3ZMH; 3ZMI; 3ZMJ; 3ZOT; 4H1D; 4HDD; 4NJN; 4NJP; 5F5B; 5F5D; 5F5G; 5F5J; 5F5K; 5MT6; 5MT7; 5MT8; 6PJ4; 6PJ5; 6PJ7; 6PJ8; 6PJ9; 6PJA; 6PJP; 6PJQ; 6PJR; 6PJU; 6VJ8; 6VJ9; 6XRO; 6XRP; |
| Mapped Pubmed ID | 17976648; 20890268; 21256137; 22515733; 22963263; 23353827; 23359682; 23609444; 23665170; 24315097; 24561554; 25009246; 26805573; 29107700; 31570873; 32888502; |
| Motif | |
| Gene Encoded By | |
| Mass | 31,307 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.105; |