| IED ID | IndEnz0002005596 |
| Enzyme Type ID | protease005596 |
| Protein Name |
Probable cytosol aminopeptidase EC 3.4.11.1 Leucine aminopeptidase LAP EC 3.4.11.10 Leucyl aminopeptidase |
| Gene Name | pepA RSc2415 RS02700 |
| Organism | Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Betaproteobacteria Burkholderiales Burkholderiaceae Ralstonia Ralstonia solanacearum (Pseudomonas solanacearum) Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) |
| Enzyme Sequence | MEFSTKALDWAKAGPSGALAAKSDCLVIGLFESQTLAGAAKALDVATKGLVGRLVKLGDFEGKRGTSLLLHEVAGVGAARVLLVGLGKEAEFTDRAYAEAVRTALRALSGTKAANVTWTLTQQPARDKDAAWAVLTAVTLIREAGYRFIERHPELKSKRDKSGGGLRKVMLTVDAADAKAAAVAAARGAAIANGMELTRDLGNLPSNICTPTYLANTARQIAKDFKLKVEVLGRKQIEALKMGAFLAVTKGSQEPPQFIVLRYEGGPAKQAPVVLVGKGITFDTGGISLKPGEGMDEMKFDMCGAASVLGTLRAVAEMGLKLNVIAVVPTCENMPSGIATKPGDVVTSMSGQTIEILNTDAEGRLILCDALTYVERFRPAAVIDVATLTGAVIIALGHINTGVYARSDALANALLAAGKQSLDTGWRMPLDEEYQELLKSNFADMGNIGGRPAASVTAACFLARFTEKYDWAHLDIAGTAWKSGAAKGATGRPVPLLTRFLMDRAG |
| Enzyme Length | 506 |
| Uniprot Accession Number | Q8XWQ8 |
| Absorption | |
| Active Site | ACT_SITE 290; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 364; /evidence=ECO:0000255|HAMAP-Rule:MF_00181 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; |
| DNA Binding | |
| EC Number | 3.4.11.1; 3.4.11.10 |
| Enzyme Function | FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Metal binding (7) |
| Keywords | Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 53,189 |
| Kinetics | |
| Metal Binding | METAL 278; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 283; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 283; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 301; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 360; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 362; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 362; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181 |
| Rhea ID | |
| Cross Reference Brenda |