| IED ID | IndEnz0002005633 |
| Enzyme Type ID | protease005633 |
| Protein Name |
Probable cytosol aminopeptidase EC 3.4.11.1 Leucine aminopeptidase LAP EC 3.4.11.10 Leucyl aminopeptidase |
| Gene Name | pepA lap sll2001 |
| Organism | Synechocystis sp. (strain PCC 6803 / Kazusa) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Cyanobacteria/Melainabacteria group Cyanobacteria Synechococcales Merismopediaceae Synechocystis unclassified Synechocystis Synechocystis sp. PCC 6803 Synechocystis sp. (strain PCC 6803 / Kazusa) |
| Enzyme Sequence | MQIRGTDYTALTWQGDALALGFFENATEITGDLTQLDDRLEGVLRELIEEKEFKGKAGQKLVSRVGSKTPIKKLILVGLGEIEKFNSQVLGDGAAAIARLAKGEKVKTLGVSLPQREHDPAQTAAILTEGILLALHQDNRFKSDPEDKEIKLTTVELLGLGEQSTAIGKAEKIVSGVILAREMVAAPANEVTPLTFTEIATELAQTYGLELEVLGQTECEALGMGAFLGVAKASELPPQFIHLTYRPANPVKKLAIIGKSLTFDSGGLNIKGAGSGIETMKMDMGGGGATLGAAKAIAQLKPNVEIHFICAATENMISGTAMHPGDILTASNGKTIEVNNTDAEGRLTLADALVFAEKLGVEAIVDLATLTGACIVALGDDIGGLWSPNQELADELKVAADKAGEKFWQMPMESKYFEGLKSPIADMKNTGPRSGGSITAALFLQQFIKETPWAHLDIAGPVWTDKQNGVHNAGATGYPVRTLVQWVLGLAE |
| Enzyme Length | 492 |
| Uniprot Accession Number | P73971 |
| Absorption | |
| Active Site | ACT_SITE 271; /evidence=ECO:0000255; ACT_SITE 346; /evidence=ECO:0000255 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; |
| DNA Binding | |
| EC Number | 3.4.11.1; 3.4.11.10 |
| Enzyme Function | FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Erroneous initiation (1); Metal binding (7) |
| Keywords | Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 18000013; |
| Motif | |
| Gene Encoded By | |
| Mass | 52,166 |
| Kinetics | |
| Metal Binding | METAL 259; /note=Manganese 2; /evidence=ECO:0000250; METAL 264; /note=Manganese 1; /evidence=ECO:0000250; METAL 264; /note=Manganese 2; /evidence=ECO:0000250; METAL 283; /note=Manganese 2; /evidence=ECO:0000250; METAL 342; /note=Manganese 1; /evidence=ECO:0000250; METAL 344; /note=Manganese 1; /evidence=ECO:0000250; METAL 344; /note=Manganese 2; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |