| IED ID | IndEnz0002005637 |
| Enzyme Type ID | protease005637 |
| Protein Name |
Probable cytosol aminopeptidase EC 3.4.11.1 Leucine aminopeptidase LAP EC 3.4.11.10 Leucyl aminopeptidase |
| Gene Name | pepA Tery_0069 |
| Organism | Trichodesmium erythraeum (strain IMS101) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Cyanobacteria/Melainabacteria group Cyanobacteria Oscillatoriophycideae Oscillatoriales Microcoleaceae Trichodesmium Trichodesmium erythraeum Trichodesmium erythraeum (strain IMS101) |
| Enzyme Sequence | MEIKVGNIPQLKWAGDALAIGLFEDAIKLTEDAIELTEEMAELDEMLSGTLSELIKETEFKGKANSSVSTRVGIKTSIRKIIVVGLGKLEKFKLDSLRQAAATCGRLAKKERCKTLGISLPMCQDADSTAIAITEGIELALHQDNRFKSEPENLGPDLEKVELIGLAASDEAIAHARKICSGVIFARELVAAPANSCTPITMAETAQTLAKEFGLTLEILEKEDCEKLGMGAFLGVAQGSDLPPKFIHVTYKPEVTPRRKLAIVGKGLTFDSGGLNLKVSGSGIEMMKIDMGGAGTTFGTIKAIAQLKPDVEVHFISAVTENMVSGHAIHPGDFLTASNGKIIEVNNTDAEGRLTLADALVFAEKLGVDAIIDLATLTGACVVALGNDIAGLWSPNDNLAAEITAAAEKAGEKMWRMPLEEKYFEGLKAMHADMKNTGPRPGGAITAALFLKQFVKNTPWAHLDIAGPVWVDTENGYNNQGATGYGVRTLVNWILS |
| Enzyme Length | 496 |
| Uniprot Accession Number | Q11A96 |
| Absorption | |
| Active Site | ACT_SITE 278; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 353; /evidence=ECO:0000255|HAMAP-Rule:MF_00181 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; |
| DNA Binding | |
| EC Number | 3.4.11.1; 3.4.11.10 |
| Enzyme Function | FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Metal binding (7) |
| Keywords | Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 52,944 |
| Kinetics | |
| Metal Binding | METAL 266; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 271; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 271; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 290; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 349; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 351; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 351; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181 |
| Rhea ID | |
| Cross Reference Brenda |