| IED ID | IndEnz0002005639 |
| Enzyme Type ID | protease005639 |
| Protein Name |
Probable cytosol aminopeptidase EC 3.4.11.1 Leucine aminopeptidase LAP EC 3.4.11.10 Leucyl aminopeptidase |
| Gene Name | pepA Ava_2727 |
| Organism | Trichormus variabilis (strain ATCC 29413 / PCC 7937) (Anabaena variabilis) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Cyanobacteria/Melainabacteria group Cyanobacteria Nostocales Nostocaceae Trichormus Anabaena variabilis Trichormus variabilis (strain ATCC 29413 / PCC 7937) (Anabaena variabilis) |
| Enzyme Sequence | MAIQLSDKPLLEWAGDTLAIALFEDAVELTGELASLDEKFAGILKELIAEEEFTGKANSTVFTRVSANIPVRKIILVGLGKTEAFNIDTLRRAAAAVGKVGKKQKSKVIGLSFPLWNNDPTASSQAIAEGVQLALYQDNRFKSDPDDKGSQVETVELLGFAGQEAAINRANQIVSGVILARQLVAAPANSVTPITMAETAQQIAQDYGLQIEILEQEDCEKLGMGAFLGVALASDLPPKFIHLTYKPESTPKRKLAIVGKGLTFDSGGLNIKGAGSGIETMKIDMGGAAATLGAAKAIAQIKPNVEVHFISAVTENMISGKAMHPGDILTASNGKTIEVNNTDAEGRLTLADALVYTDKLGLDAIVDLATLTGANVIALGDDIAGLYTPDDALAGQLEQAASESGEKIWRMPLEEKYFEGLKSGIADMKNTGPRPGGSITAALFLKQFVKDTPWAHLDIAGPVWADKENGYNGPGATGYGVRLLVDWVLSE |
| Enzyme Length | 491 |
| Uniprot Accession Number | Q3M9J6 |
| Absorption | |
| Active Site | ACT_SITE 272; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 347; /evidence=ECO:0000255|HAMAP-Rule:MF_00181 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; |
| DNA Binding | |
| EC Number | 3.4.11.1; 3.4.11.10 |
| Enzyme Function | FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Metal binding (7) |
| Keywords | Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 51,902 |
| Kinetics | |
| Metal Binding | METAL 260; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 265; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 265; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 284; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 343; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 345; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 345; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181 |
| Rhea ID | |
| Cross Reference Brenda |