| IED ID | IndEnz0002005660 |
| Enzyme Type ID | protease005660 |
| Protein Name |
Probable cytosol aminopeptidase EC 3.4.11.1 Leucine aminopeptidase LAP EC 3.4.11.10 Leucyl aminopeptidase |
| Gene Name | pepA WS1353 |
| Organism | Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria delta/epsilon subdivisions Epsilonproteobacteria Campylobacterales Helicobacteraceae Wolinella Wolinella succinogenes Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes) |
| Enzyme Sequence | MEILCHSAPLHEIKAEASLIFVINKEFSHEWIADSELFGSVGFEGKEGQTLFIPQSKTLYYALDSLTPDSIRESSAKALRALKKYPFSSFKVGIYTQESLECLEAMAAGFLLGDYNFETYKSQKSESKLTQILISLQTHLGRQIDSKSFETILARQSAIARAVNLARELVNTPPEDATPQKIAQAAQKVAQEHQLECRIEDEKFLEKEQMGAFLAVSRASIHRPRLIHLTHQSPKPKLKVALVGKGLTYDSGGLSLKPADFMVTMKADKGGGCAVIAILQAAKELGLELELHGIVGATENMIGGNAYKPDDVLRAKNGKTIEIRNTDAEGRLVLADCLVYAQELKPDYIIDLATLTGACVVGLGEYTSGVLGHSQELKHRFIECATQSGELTADLPFNRHLKKLIESKVADVCNVSSTRYGGSITAGLFLSEFIEESYKEKWLHLDIAGPAYVEKEWDVNPHGASGAGVRMVLQFLEQLTKES |
| Enzyme Length | 483 |
| Uniprot Accession Number | Q7M8W6 |
| Absorption | |
| Active Site | ACT_SITE 257; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 331; /evidence=ECO:0000255|HAMAP-Rule:MF_00181 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; |
| DNA Binding | |
| EC Number | 3.4.11.1; 3.4.11.10 |
| Enzyme Function | FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Metal binding (7) |
| Keywords | Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 53,076 |
| Kinetics | |
| Metal Binding | METAL 245; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 250; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 250; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 268; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 327; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 329; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 329; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181 |
| Rhea ID | |
| Cross Reference Brenda |