| IED ID | IndEnz0002005680 |
| Enzyme Type ID | protease005680 |
| Protein Name |
Apoptotic protease-activating factor 1 APAF-1 |
| Gene Name | apaf1 |
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio) |
| Enzyme Sequence | MEERARSRLLRSKATLEQDIKASYLMDHMISDGVLTNDEEAKVLSKATRKEQAVALLETLLRKDNRAYISFYNALIRESYGDLASLLHSDLPLLSPEGEKSFADGVSPSVQAILSVGGVPQRPVVFVSRPPLLNLIREMLYQLRDTPGWVTVFGMAGSGKSVMAAEVVRDRSLIKECFPDGVHWLSVGQCERADLLVRMQSLCFRLEQCQSSDTSQRPPSTVEEAKERLRFLMLRRFPRSLLILDDVWDSSSLRSFDIQCRVLLTTRNRALTDSVSGVRYEVPVENGLDEEKALEILALYVNGKMHKLPEQARSIVSECKGSPLVVSLIGALLREFPDRWSYYLRQLQQKQFKRIRKSSSYDYEALDQAMDASLQVLEAEHQELYRDLSVMQKDIKVPAKVLSVLWGLELEEVEDVLQEFVNKSLLFRDCNQRPYRYYLHDLQLDFLAEQNRDQIAELHKKMVRQYQRFYSKRPPDSADKDSLYWYQFIPYHMAKAGLSKELYSLMFSLDWVKEKARIMGSAHLINDYVEYGEILDKENSEVRVQFQEFLSLNGHHLEQRPFPDVVQLALSQPDRSEVYRQALMQAQKRASRGQIYLNWVNKNIEEGLSRLVMHPHQGAVYYACFSKDGSKIASCGASKALRVFKSTSGEKLLELQAHEEDVLCCAFSPDDRHIATCASDRKVKLWNVERGVLIREFEVEHEEQINHCQFTNTGRRVLLATCSNDKFTNTRLWNPNKKTSQNTMFGHMEPVNHCCFSPNDLYLATSSSDGSLKLFEVSSANEWKSIDVDSFFPESDEEIKAMVKCSTWSADGSQIICAARNTVFVFDVETSDLLLKLKTSRLSTIQFCHACPNSSLLAVALSHYTVELWNFESSKKKAECSGHLSWVHCVQFSPDGSLLLSSSDDQTIRLWETDRVHTSSAVALKRDTDVLSSHSDATIIAPDSSNRLQVLSGSTGAVVLESEELSSRIRCSCISRNAAFVALGSEDGTVQVIEVPSSKASVKLSGHTKTVHHCQFTDDCEILITSSEDSTIRVWKWRTGECMVLQGHMEPVRKFHLLSSSSSPHLFSWSFDGTVKVWDLTRGQMLQDLVCHEGAVLSCDVSSDGRLFATTSANRTAKVWSSASWKMLFLLEGHKDCVRSCRFSWDNKRLATGDDNGEIRLWSMLDGALLKICPRDTKDSMNSYHAGWVTDLHFSPDNRVLVSTAGYIKWWSVESGEALQTFYTMGGNLKKIHVSPDFSTFITVDSIGILYILKRLEGEGT |
| Enzyme Length | 1261 |
| Uniprot Accession Number | Q9I9H8 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 154..161; /note=ATP; /evidence=ECO:0000255 |
| Features | Chain (1); Domain (2); Nucleotide binding (1); Repeat (13) |
| Keywords | ATP-binding;Apoptosis;Cytoplasm;Nucleotide-binding;Reference proteome;Repeat;WD repeat |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 142,934 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |