| IED ID | IndEnz0002005698 |
| Enzyme Type ID | protease005698 |
| Protein Name |
ATPase histone chaperone YTA7 EC 3.6.1.- ATPase family AAA domain-containing protein YTA7 AAA-ATPase Tat-binding homolog 7 |
| Gene Name | YTA7 YGR270W |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MARNLRNRRGSDVEDASNAKVGYETQIKDENGIIHTTTRSLRKINYAEIEKVFDFLEDDQVMDKDETPVDVTSDEHHNNNQKGDDEDDDVDLVSPHENARTNEELTNERNLRKRKAHDPEEDDESFHEEDVDDDEEEEEADEFEDEYLDEDSKDNNRRRRAADRKFVVPDPDDDEEYDEDDEEGDRISHSASSKRLKRANSRRTRSSRHPETPPPVRRALRSRTRHSRTSNEENDDENDNSRNEALTLADEIRELQEDSPIREKRFLRERTKPVNYKLPPPLTASNAEEFIDKNNNALSFHNPSPARRGRGGWNASQNSGPTRRLFPTGGPFGGNDVTTIFGKNTNFYNQVPSAFSDNNNNKLILDSDSSDDEILPLGVTPKTKKENTQKKKKKKPEIADLDPLGVDMNVNFDDIGGLDNYIDQLKEMVALPLLYPELYQNFNITPPRGVLFHGPPGTGKTLMARALAASCSSDERKITFFMRKGADILSKWVGEAERQLRLLFEEAKKHQPSIIFFDEIDGLAPVRSSKQEQIHASIVSTLLALMDGMDNRGQVIVIGATNRPDAVDPALRRPGRFDREFYFPLPDVKARFKILQIQTRKWSSPLSTNFIDKLAFLTKGYGGADLRSLCTEAALISIQRSFPQIYRSNDKLLVDPSKIKVKVSDFMLALKKIVPSSARSTGSSPQPLPELIKPLLADQLNNLKNKLDYMLNIKDTTFQRNTSLLQNFIDYEEYSGEEEEHDKYGGNEDTSSFRSYEFFESMAESQICKPRLLINGPKGNGQQYVGAAILNYLEEFNVQNLDLASLVSESSRTIEAAVVQSFMEAKKRQPSVVFIPNLDIWINTIPENVILVLSGLFRSLQSNEKILLLCLAENLDISEVKNGILSDFAFDKNIFQLHKPSKENITRYFSNLIELLKTKPSDIPMKKRRVKPLPELQKVTSNAAPTNFDENGEPLSEKVVLRRKLKSFQHQDMRLKNVLKIKLSGLMDLFKNRYKRFRKPPIDDAFLVHLFEPETSNDPNWQPAYIKDENMILEVSTGRKFFNMDLDIVEERLWNGYYSEPKQFLKDIELIYRDANTIGDRERVIKASEMFANAQMGIEEISTPDFIQECKATRQRDLERQELFLEDEEKRAAMELEAKEQSQENILQEPDLKDNKANEFGVAAGNQLQAQLQTTINTASIVNNSEVPQPIDTNLYKKEIPAAIPSAVDKEKAVIPEDSGANEEYTTELIQATCTSEITTDDDERARKEPKENEDSLQTQVTEENFSKIDANTNNINHVKEIQSVNKPNSLHETVEKRERSPIPKEVVEPEQGKKSDKELILTPEQIKKVSACLIEHCQNFTVSQLEDVHSSVAKIIWKSKSAWDKTGTVDEIIKFLSE |
| Enzyme Length | 1379 |
| Uniprot Accession Number | P40340 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.6.1.- |
| Enzyme Function | FUNCTION: Functions as an ATP-dependent nucleosome disassembly factor that helps evict canonical histone H3 from the 5'-end of genes upon their induction (PubMed:25406467). Also contributes to kinetochore assembly by cooperating with SCM3 to load the histone H3 variant CSE4/CENP-A at centromeres (PubMed:32079723). Provides a chromatin boundary function at the 5'-end of genes that restricts access by RTT106 and thus prevents ectopic spreading of repressive chromatin into coding regions (PubMed:19683497, PubMed:25406467, PubMed:22156209). Also prevents heterochromatin spreading downstream of the silent mating-type locus HMR, this function is independent of the tRNA boundary element (PubMed:16079223). Contributes to appropriate cell cycle regulation of histone gene expression by recruiting RNA polymerase II to histone genes, and subsequent CDK1- and casein kinase II-dependent eviction from chromatin is required to promote transcriptional elongation (PubMed:22156209). {ECO:0000269|PubMed:16079223, ECO:0000269|PubMed:19683497, ECO:0000269|PubMed:22156209, ECO:0000269|PubMed:25406467, ECO:0000269|PubMed:32079723}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 454..461; /note=ATP; /evidence=ECO:0000255 |
| Features | Chain (1); Compositional bias (8); Domain (1); Initiator methionine (1); Modified residue (15); Mutagenesis (14); Nucleotide binding (1); Region (7); Sequence conflict (8) |
| Keywords | ATP-binding;Acetylation;Bromodomain;Centromere;Chromosome;Hydrolase;Nucleotide-binding;Nucleus;Phosphoprotein;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Chromosome, centromere {ECO:0000269|PubMed:32079723}. Nucleus {ECO:0000269|PubMed:32079723}. Chromosome {ECO:0000269|PubMed:19683497, ECO:0000269|PubMed:22156209, ECO:0000269|PubMed:25406467}. Note=Binds near the 5'-end within protein-coding genes (PubMed:25406467, PubMed:19683497). Localizes to the negative regulatory element NEG and promoter of the HTA1 gene during G2 and M-phase, and its open reading frame during G1/S (PubMed:22156209, PubMed:19683497). {ECO:0000269|PubMed:19683497, ECO:0000269|PubMed:22156209, ECO:0000269|PubMed:25406467}. |
| Modified Residue | MOD_RES 2; /note="N-acetylalanine"; /evidence="ECO:0007744|PubMed:17287358"; MOD_RES 11; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:17287358, ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198"; MOD_RES 17; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:17287358"; MOD_RES 94; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:17330950"; MOD_RES 212; /note="Phosphothreonine"; /evidence="ECO:0007744|PubMed:18407956"; MOD_RES 229; /note="Phosphothreonine"; /evidence="ECO:0007744|PubMed:19779198"; MOD_RES 241; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:18407956"; MOD_RES 259; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198"; MOD_RES 285; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:18407956"; MOD_RES 367; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:19779198"; MOD_RES 369; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:19779198"; MOD_RES 370; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:19779198"; MOD_RES 735; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:19779198"; MOD_RES 1142; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198"; MOD_RES 1256; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198" |
| Post Translational Modification | PTM: Phosphorylated by CDK1 and casein kinase II during S-phase, which leads to its eviction from histone gene promoters and promotes histone gene transcription. {ECO:0000269|PubMed:22156209}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11805826; 11805837; 12702274; 14574415; 14690591; 15824130; 15972895; 16429126; 17082210; 17107617; 17360454; 18202364; 18467557; 18493054; 19034519; 19106085; 19416104; 19536198; 19581291; 20107600; 20489023; 20699646; 21179020; 21326895; 21670546; 22074782; 22199229; 22345607; 22555441; 22912562; 22932476; 23974242; 24459729; 25377252; 25786853; 26602697; 27693354; |
| Motif | |
| Gene Encoded By | |
| Mass | 157,407 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |