| IED ID | IndEnz0002005742 |
| Enzyme Type ID | protease005742 |
| Protein Name |
Gag polyprotein Pr53Gag Cleaved into: Matrix protein p19 MA ; Capsid protein p24 CA ; Nucleocapsid protein p15-gag NC-gag |
| Gene Name | gag |
| Organism | Human T-cell leukemia virus 1 (strain Japan ATK-1 subtype A) (HTLV-1) |
| Taxonomic Lineage | Viruses Riboviria Pararnavirae Artverviricota Revtraviricetes Ortervirales Retroviridae Orthoretrovirinae Deltaretrovirus Primate T-lymphotropic virus 1 Human T-cell leukemia virus type I HTLV-1 subtype A Human T-cell leukemia virus 1 (strain Japan ATK-1 subtype A) (HTLV-1) |
| Enzyme Sequence | MGQIFSRSASPIPRPPRGLAAHHWLNFLQAAYRLEPGPSSYDFHQLKKFLKIALETPARICPINYSLLASLLPKGYPGRVNEILHILIQTQAQIPSRPAPPPPSSPTHDPPDSDPQIPPPYVEPTAPQVLPVMHPHGAPPNHRPWQMKDLQAIKQEVSQAAPGSPQFMQTIRLAVQQFDPTAKDLQDLLQYLCSSLVASLHHQQLDSLISEAETRGITGYNPLAGPLRVQANNPQQQGLRREYQQLWLAAFAALPGSAKDPSWASILQGLEEPYHAFVERLNIALDNGLPEGTPKDPILRSLAYSNANKECQKLLQARGHTNSPLGDMLRACQTWTPKDKTKVLVVQPKKPPPNQPCFRCGKAGHWSRDCTQPRPPPGPCPLCQDPTHWKRDCPRLKPTIPEPEPEEDALLLDLPADIPHPKNSIGGEV |
| Enzyme Length | 429 |
| Uniprot Accession Number | P03345 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: [Gag polyprotein]: The matrix domain targets Gag, Gag-Pro and Gag-Pro-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. {ECO:0000269|PubMed:15476809}.; FUNCTION: [Matrix protein p19]: Matrix protein. {ECO:0000269|PubMed:15476809}.; FUNCTION: [Capsid protein p24]: Forms the spherical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. {ECO:0000305}.; FUNCTION: [Nucleocapsid protein p15-gag]: Binds strongly to viral nucleic acids and promote their aggregation. Also destabilizes the nucleic acids duplexes via highly structured zinc-binding motifs. {ECO:0000269|PubMed:25686502}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (1); Chain (4); Compositional bias (1); Disulfide bond (1); Helix (11); Initiator methionine (1); Lipidation (1); Modified residue (1); Motif (2); Mutagenesis (5); Region (1); Sequence conflict (2); Site (2); Turn (4); Zinc finger (2) |
| Keywords | 3D-structure;Capsid protein;Direct protein sequencing;Disulfide bond;Host-virus interaction;Lipoprotein;Metal-binding;Myristate;Phosphoprotein;Reference proteome;Repeat;Ribosomal frameshifting;Ubl conjugation;Viral nucleoprotein;Virion;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Matrix protein p19]: Virion {ECO:0000269|PubMed:15476809}.; SUBCELLULAR LOCATION: [Capsid protein p24]: Virion {ECO:0000305}.; SUBCELLULAR LOCATION: [Nucleocapsid protein p15-gag]: Virion {ECO:0000305}. |
| Modified Residue | MOD_RES 105; /note=Phosphoserine; by host MAPK1; /evidence=ECO:0000269|PubMed:16635502 |
| Post Translational Modification | PTM: [Gag polyprotein]: Specific enzymatic cleavages by the viral protease yield mature proteins. The polyprotein is cleaved during and after budding, this process is termed maturation. {ECO:0000269|PubMed:2843670, ECO:0000269|PubMed:6280175}.; PTM: [Matrix protein p19]: Phosphorylation of the matrix protein p19 by MAPK1 seems to play a role in budding. {ECO:0000269|PubMed:16635502}.; PTM: [Gag polyprotein]: Ubiquitinated by host NEDD4. {ECO:0000269|PubMed:15126635}.; PTM: [Gag polyprotein]: Myristoylated. Myristoylation of the matrix (MA) domain mediates the transport and binding of Gag polyproteins to the host plasma membrane and is required for the assembly of viral particles. {ECO:0000269|PubMed:11333909, ECO:0000269|PubMed:1398108}. |
| Signal Peptide | |
| Structure 3D | NMR spectroscopy (1) |
| Cross Reference PDB | 1QRJ; |
| Mapped Pubmed ID | - |
| Motif | MOTIF 118..121; /note=PPXY motif; /evidence=ECO:0000269|PubMed:15126635; MOTIF 124..127; /note=PTAP/PSAP motif; /evidence=ECO:0000269|PubMed:15126635 |
| Gene Encoded By | |
| Mass | 47,496 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |