| IED ID | IndEnz0002005747 |
| Enzyme Type ID | protease005747 |
| Protein Name |
Lectizyme EC 3.4.21.- Proteolytic lectin |
| Gene Name | Gpl |
| Organism | Glossina austeni (Savannah tsetse fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Calyptratae Hippoboscoidea Glossinidae Glossina (tsetse flies) Glossina Glossina austeni (Savannah tsetse fly) |
| Enzyme Sequence | MKFFAVFALCVASVSAANLDAIAKPGFPAGRIINGHEAEKGEAPFIVSLKAGKGHFCGGSIIAENWVLTAGHCLIFDEFEIVAGLHSRNDESDVQIRKVTGKHQQIVHEKYGGGVGPNDIGLIYVDKPFNLNALTRDGTAAVAKVNLPTGKYESTGEGKLYGWGLDNSGFSPNILNTLDVNIIGYEECKNALNSDAPLDPVNICSYTAGAIDGACNGDSGGPMVRITPDGTELVGIVSWGYQPCASTTMPSVYTWTSAFDKWIEDSIENYAQLL |
| Enzyme Length | 274 |
| Uniprot Accession Number | Q4TTV7 |
| Absorption | |
| Active Site | ACT_SITE 72; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 119; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 219; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Protein with lectin and protease activity involved in the establishment of trypanosome infections in tsetse flies. Binds D-glucosamine and agglutinates bloodstream-form trypanosomes and rabbit red blood cells. Capable of inducing transformation of bloodstream-form trypanosomes into procyclic (midgut) forms in vitro. {ECO:0000269|PubMed:16619615}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (3); Domain (1); Signal peptide (1) |
| Keywords | Disulfide bond;Hydrolase;Protease;Secreted;Serine protease;Signal |
| Interact With | |
| Induction | INDUCTION: By blood meal. {ECO:0000269|PubMed:16619615}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16619615}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 29,151 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |