| IED ID | IndEnz0002005756 |
| Enzyme Type ID | protease005756 |
| Protein Name |
Probable TtuB-protein conjugate cleaving protease EC 3.4.19.- |
| Gene Name | TT_C1133 |
| Organism | Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Deinococcus-Thermus Deinococci Thermales Thermaceae Thermus Thermus thermophilus Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) |
| Enzyme Sequence | MAPPSVPIVYAGGRGGCPGGYHGLVLYVPRGLLEETRAHLLREAPKEGVGLWAGRREVERVIPLPNVHPSPLTAYLADPLALLKALKALEREGLSLLAIYHSHPKGPALPSPRDIKEARWRVPYVIFGTDGVRAFLLPEGQEVALVVL |
| Enzyme Length | 148 |
| Uniprot Accession Number | Q72IJ9 |
| Absorption | |
| Active Site | ACT_SITE 47; /note=Proton donor/acceptor; /evidence=ECO:0000250|UniProtKB:D4GTS4 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.19.- |
| Enzyme Function | FUNCTION: Probable metalloprotease that cleaves the ubiquitin-like modifier protein TtuB from protein conjugates, hydrolyzing the isopeptide bond between a lysine residue of the target protein and the C-terminal glycine of the modifier protein. Does not seem to work for all the TtuB conjugates. {ECO:0000305|PubMed:22467871}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Domain (1); Metal binding (3); Motif (1); Mutagenesis (2); Site (1) |
| Keywords | Hydrolase;Metal-binding;Metalloprotease;Protease;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 101..114; /note="JAMM motif"; /evidence="ECO:0000250|UniProtKB:D4GTS4, ECO:0000305|PubMed:22467871" |
| Gene Encoded By | |
| Mass | 16,032 |
| Kinetics | |
| Metal Binding | METAL 101; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:D4GTS4; METAL 103; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:D4GTS4; METAL 114; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:D4GTS4 |
| Rhea ID | |
| Cross Reference Brenda |